4810860
This presentation is the property of its rightful owner.
Sponsored Links
1 / 77

دکترشهلا فارسي PowerPoint PPT Presentation


  • 142 Views
  • Uploaded on
  • Presentation posted in: General

دکترشهلا فارسي. مديرايمني وبهداشت آزمايشگاه مرجع سلامت. دبيرکميته کشوري ايمني وامنيت زيستي آزمايشگاهي. Occupational infections USA > 10 000 (1983) < 400 (2001). Laboratory acquired infections, LAI. Biosafety Management. SARS 2003-04: 4 lab staff, 7 secondary cases

Download Presentation

دکترشهلا فارسي

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript



Occupational infections USA> 10 000 (1983) < 400 (2001)


Laboratory acquiredinfections, LAI

Biosafety Management

  • SARS 2003-04: 4 lab staff, 7 secondary cases

  • Ebola 2004: 46-y women, dead, Russia

  • Tb 2004: 3 cases from leaky aerosol chamber, USA

  • Legionella 2003: pneumonia in BMA, SMI, Sweden

  • Cutaneous antrax 2002: lab technician, Texas

  • Vaccinia 2002: 26-y lab technician,

  • Brazil

  • West Nile virus 2002: 2 cases, USA

  • Melioidosis 2000: microbiologist, USA

  • Herpes B encephalitis 1997: 23-y woman, dead



Elements of Safety Program

Safety policy

Safety officer

Safety manual


Elements of Safety Program

Safety oriented

employees

Safety committee

Safetytraining

Accident report


Elements of Safety Program

Incorporate safety

into procedures

Safety checklist

(Continious improvement)

Documentation


Safety Management

  • ( )

  • Management responsibilities:

  • :


  • Officer) (Safety


Safety Officer

:

  • ( ..)


  • ..


  • :

  • (Safety Officer)



( ) .

.

.

.



General Requirements for Personal and Laboratory safety

  • HIV .


General Requirements for Personal safety

  • .

  • .

  • .


General Requirements for Personal safety

  • . .

  • 30-12


General Requirements for Personal safety

  • .

  • .

  • .

  • 16 .


General Requirements for Personal and Laboratory safety

  • .

  • .

  • .

  • .


  • .

  • .... .

  • ... .


Personal Protective Equipment(PPE)


  • .

  • - -

  • ( )


  • .

  • ( )


... .

.


Personal protective equipment

Eye protectors

  • Safety glasses with side protection

  • Face shield (visor); two types


  • .


Personal protective equipment

Respirators with HEPA

Full- or half facepiece respirators

(replaceable filters)

Decontamination; protection from particles and gas

Particulate respirators

(mask with filter)

Protection particles; FFP1-FFP3

Powered respirators (replaceable filters, FFP1-FFP3)

Airflow at min. 120 l/minute for protection


  • .

  • .

    ( )


.



  • Risk group1- 4

    Biosafety level = BSL1- 4

    Biosafety cabinet = BSC class 1- 3

    : Risk Management,

    Risk Assessment

    .........


1

2

3

4

Foot and mouth

Disease virus

Ebola virus

Lassa virus

Variola virus

Risk Groups

Hepatitis C virus

HIV

New castle disease

Yellow fever

Monkey pocks virus

Lactobacillus bulgaricus (yoghurt)

-Phage

Tobacco mosaic virus

Polio myelitis virus

Rhino virus

Vaccinia virus

Polio virus

Vibrio cholerae

Biosafety Levels

4

2

1

3


Biosafety Cabinet of Class III


Biosafety Level 4




  • 1/10 .( 5% )


  • B


  • B -

    ( ) ( ) .

  • .



Laboratory hazards


Route of Exposure or Mode of Entry

  • ( )


Route of Exposure orMode of Entry

  • ( )

  • ()


Aerosols are generated from most laboratory tasks


Aerosols can spread through a building and effect many people


Contamination is often heaviest in work areas and on worker hands


Loop microincinerators, disposable loops


Episodes of Single-source, Multiple Laboratory Infections




  • .( 15 30 ) .

  • .

  • .

  • .


  • .

  • .( )

  • ( Safety Box) .

  • .

  • : 70% ...



What is wrong and what would you do in these cases?




  • Cleaning))

  • :

  • :

  • () : (180-160 4-2h)

  • ( )-

  • c121- 30


  • :

  • ( TST=Time, Steam,Tempreture )

  • :


Efficacy testing of autoclaves

  • Chemical indicators (EN 867):

  • Results directly after the process

  • For 121C and 134

  • Cheap and easy

  • Treatment control / endpoint control

  • Disadvantage: only limited temperature and

  • time details

Color change

integrator


Efficacy testing of autoclaves

Biological dicators

Media with colour indicator

Spore strip with Bac. stearothermophilus (ca. 106)

processed

unprocessed

positive control


()

0/5 = %1/10 = 5

1 /0 = % 1/50 = 1


Disinfection

  • : 70%( ..)-2/3 +1/3 70%

  • 10 3

  • 5%

  • 5%-2%

  • ()10%

  • 30 %(5/1 10/1 ) - 6 % 30 - 3% - -



.

.

- - - - -


  • :

    -

    -

    - (Treatment)

    -

    -

    -

    -

    - ( )


  • Box) (Safety


  • Safety Box

  • ( )


  • - - 52- ( )- : 8 -88894445 3-88900942 88891209

    - : 66400365 :66413758 ( )

    - - 131- - 66402992()

  • - 88013960-88029527

    - 88500481


  • Login