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Help: Strain Page Header

Help: Strain Page Header. Gene/Protein : The established name for this ORF, if available. Yeast ORF deletion: _d suffix : dubious ORF _p suffix : putative (uncharacterized) ORF. SGD Linkout : Link to the SGD page for this ORF. Yeast ORF deletion: _d suffix : dubious ORF

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Help: Strain Page Header

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  1. Help: Strain Page Header Gene/Protein: The established name for this ORF, if available Yeast ORF deletion: _d suffix : dubious ORF _p suffix : putative (uncharacterized) ORF SGD Linkout: Link to the SGD page for this ORF Yeast ORF deletion: _d suffix : dubious ORF _p suffix : putative (uncharacterized) ORF Description : A description of the protein/gene from SGD Zygosity : Whether the deletion strain has been screened in a heterozygous (HIP) or homozygous (HOP) background.

  2. Help: Strain Fitness Profile • Fitness Plot: Expanded View • Fitness axis expands to cover full range of data • Fitness Plot: Fixed View • Fitness axis covers a fixed range (-5 to 12.5) • Data points outside fixed range are captured in upper/lower rescaled areas (in grey) Fitness Axis Fitness value for this deletion strain under the given screening conditions View Selector Fixed or Expanded View Growth Condition Axis Screen Condition, arranged numerically by SGTC_ID Significant Fitness Defect Scores Highlighted in Yellow. These are clickable to show details on the screen and a link to the screen profile • Information Bubble • SGTC Screen Identifier, with link to Screen Profile • Screening conditions for this point • Fitness Defect Score • P-value

  3. Help: Strain Fitness TableThis table provides details on the screens which generated high fitness defect scores.The top 20 are listed, ranked by fitness defect score. • Screen Details • Rank : By FD Score, and shown on the plot • Screen ID : The SGTC_ID assigned to this screen • Condition : Compound (and other conditions) • FD Score : the FD score for this deletion strain under the given condition • Conc : the concentration of compound screened. • P-value : from the distribution of fitness scores from all screens for this deletion strain • Strain Behaviour within screen • Rank : By FD Score, the rank of this strain among all strains in the given screen conditions. • Screen P-value : from the distribution of all fitness scores for all strains in the given screen conditions. • Screen Clearance : see methods for details. Access to full data set Either as a tab-delimited text file, or excel table.

  4. Help: Cofitness TableThis table provides details on the deletion strains with similar patterns of inhibition across screens. The top 15 with positive correlations are listed, ranked by p-value. Access to full data set Either as a tab-delimited text file, or excel table. • Cofit Details • Correlation : The correlation score calculated between fitness defects scores across screens for the query strain and this deletion strain. See methods for details on calculation. • pval : see methods for details • ORF : the ORF deleted • Gene : the associated Gene/Protein. Links to the fitness profile for this deletion strain. • Zygosity : heterozygous (HIP) or homozygous (HOP) • Description : A description of the gene, from SGD

  5. Help: Screen Page Header FDA approved indicator Screened Compound (and conditions) Often a library ID. Links to pubchem IUPAC name Compound Description When available MeSH terms, from PubChem • Screen Information: • Exp ID : unique SGTC identifier • Concentration • Source : Library Source for this compound • CID : Links to pubchem • SMILES string • Molecular Weight • ALogP : Partition coefficient • H-bond Donor/Acceptor Counts • Relevant Links • PubChem : http://pubchem.ncbi.nlm.nih.gov/ • DrugBank : http://www.drugbank.ca/ • Wikipedia : http://en.wikipedia.org/ • WikiGenes : http://www.wikigenes.org/ • ChEMBL : https://www.ebi.ac.uk/chembl/ • ChemSpider : http://www.chemspider.com/ Chemical Structure Image Pool Growth Kinetics: Relative growth inhibition, calculated separately for the homozygous and heterozygous deletion pools grown under these screening conditions vs control conditions

  6. Help: Screen Fitness Profile • Fitness Plot: Expanded View • Fitness axis expands to cover full range of data • Fitness Plot: Fixed View • Fitness axis covers a fixed range (-5 to 12.5) • Data points outside fixed range are captured in upper/lower rescaled areas (in grey) Profile Type HIP : heterozygous strains HOP : homozygous strains Fitness Axis Fitness value for this deletion strain under the given screening conditions View Selector Fixed or Expanded View • Information Bubble • Gene Symbol + ORF, with link to strain profile • Fitness Defect Score • P-value • Clearance Value • Gene Description Deletion Strain Axis Arranged alphabetically by Gene name Significant Fitness Defect Scores (labelled with gene) Highlighted in Yellow. These are clickable to show details on the screen and a link to the strain profile HIP Target Highlighted in red. Determined to be a target for this screening compound. See methods. Frequent Flyer Marked with a cross. Deletion strain is frequently seen with a fitness defect across screens . Access to full data set Either as a tab-delimited text file, or excel table.

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