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The Introduction of the theory in GFFF ,mainly about the HLF(hydrodynamic lift force) and viscosity influence. Lvbo 20120411. Introduce. The FFF(field flow fractionation):the concept of field and flow . GFFF: Particles >1 µm. Applicable to water and organic solvents

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The Introduction of the theory in GFFF ,mainly about the HLF(hydrodynamic lift force) and viscosity influence

Lvbo

20120411


Introduce
Introduce HLF(hydrodynamic lift force) and viscosity influence

The FFF(field flow fractionation):the concept of fieldand flow.

GFFF:

Particles >1 µm. Applicable to water and organic solvents

Mode: Steric、Hyperlayer(Focus)、Brown

Advantage:

The simplest design of all FFF methods

The cheapest and the most basic

Constant gravity field

Do not damage to the sample and keep

the integrity of the structure and function of them

30cm~50cm

Inject

2cm


Application of gfff
Application of GFFF HLF(hydrodynamic lift force) and viscosity influence

micrometer-sized particles: coal particles,silica particles,clay particles,starch granules,yeast,and pollens.

Biological: lymphocytes,red blood cells,parasites,stem cells,and bacteria…….


Theory
Theory HLF(hydrodynamic lift force) and viscosity influence

200um

雷诺系数<2000 Parabolic flow shape

r

δ

X=r+ δ


Theory1
Theory HLF(hydrodynamic lift force) and viscosity influence

Steric:

Hyperlayer(Focus)

Brown


Theory2
Theory HLF(hydrodynamic lift force) and viscosity influence

  • Research of HLF theory:角度、流体溶液密度、流速、通道厚度。Have been discussed.

  • Chip


Theory3
Theory HLF(hydrodynamic lift force) and viscosity influence

ρm=1000 kg/m3

V=4/3*3.14*a3 m3

ν0=0.833/100 m/s (2ml/min)

r =6*10-6 m

w=200*10-6 m

c=0.0075

η=1.01*10-3 Pa*s


The theory of hlf
The theory of HLF HLF(hydrodynamic lift force) and viscosity influence


The theory of hlf1
The theory of HLF HLF(hydrodynamic lift force) and viscosity influence

Flw 11um~31um

Fwall 6um~12um

<2


The theory of hlf2
The theory of HLF HLF(hydrodynamic lift force) and viscosity influence

x=6.92275*10-6

Fnet=0

1um


Analysis
Analysis HLF(hydrodynamic lift force) and viscosity influence

Stress Analysis,then theory contains G、HLF、and so on.

From v to R or inverse.

Change conditions to test the S or H and resolution.Both theory and the actual.

1.Drag or Adsorption.

2.HLF(Make γ>1)

3.Rotate when flowing

<0.5

<0.005

>=0.5


Selectivity s
Selectivity: HLF(hydrodynamic lift force) and viscosity influenceS

1

2

3

d 与tr 成反比,与R成正比

假设:

即:

假设不成立

凡是由与R成比例的都可以拿来研究其选择性,如:r、x/w、Used以及其它组合等等。


H HLF(hydrodynamic lift force) and viscosity influence

H实测值:

H理论值:

扩散(布朗运动,热运动)

粒子多分散性 sample poly-dispersity

边缘效应 edge effects

非平衡效应 nonuniformities

进样体积 sample volume

柱后效应 extrachannel volumes

终端效应 channel end

。。。。。。


r=6 um HLF(hydrodynamic lift force) and viscosity influence、w=200um、v02ml/min、254nm。。。。。。


Retention ratio r
Retention ratio:R HLF(hydrodynamic lift force) and viscosity influence

1.Both up and down mode ,the R gradually increased with the channel angle for each of the carrier liquids.

2.Ideal steric FFF:R=6r/w(0.18)

only in the case of B1, the lowest viscosity solution do the observed

R <=0.18 and γ <1.

3. There does no significant difference in R for upward and downward

4.The different R of A and B indicates that viscosity is not the only factor influencing lift force. The

lower ionic strength of A for higher R .

UP

Down


H HLF(hydrodynamic lift force) and viscosity influenceB


H HLF(hydrodynamic lift force) and viscosity influenceB


H actual
H actual HLF(hydrodynamic lift force) and viscosity influence

A(HMPC)

UP

A(HMPC)

DOWN

B(HMPC+FL+NaN3)UP

B

DOWN


H sample volume sample polydispersity
H HLF(hydrodynamic lift force) and viscosity influencesample volume, sample polydispersity

H inj=0.0026 cm

The polydispersity contributions are similarly small as H inj and HB

HB=0.0020~0.013 cm


1.As the carrier viscosity increases,HLF increase,so the HLF(hydrodynamic lift force) and viscosity influenceR increasse for both A and B system.

2. The important observation is that analysis times decrease without loss of resolution.

3. Peak symmetry improves(HLF lifting of the particles away from the channel wall),so the resolution do not decrease very much.


Conclusion
Conclusion HLF(hydrodynamic lift force) and viscosity influence

H

S

1.

σ

η

HLF

R

r

Cosθ

2. The zone broadening is apparently not strongly influenced by carrier

viscosity so that the analysis times may be reduced without loss of resolution.

3. the contributions to H due to sample volume, sample polydispersity, and Brownian motion have been shown to be small compared to

the measured plate heights.


Thank You ! HLF(hydrodynamic lift force) and viscosity influence