Production of human proteins
Download
1 / 11

Production of Human Proteins - PowerPoint PPT Presentation


  • 85 Views
  • Uploaded on

Production of Human Proteins. Avery Grant and Eliza Tarwater. Diabetes- Type 1 Diabetes Mellitus. Metabolic disease Cause: lack of insulin Treatment: insulin injection. Treatment. Older. Newer. Pancreatic tissue from pigs and cattle Expensive Side effects Often contaminated.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about ' Production of Human Proteins' - hume


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
Production of human proteins
Production of Human Proteins

Avery Grant and Eliza Tarwater


Diabetes type 1 diabetes mellitus
Diabetes- Type 1 Diabetes Mellitus

  • Metabolic disease

    • Cause: lack of insulin

    • Treatment: insulin injection


Treatment
Treatment

Older

Newer

  • Pancreatic tissue from pigs and cattle

    • Expensive

    • Side effects

    • Often contaminated

  • GM Human Insulin

    • Genetically modified bacteria and yeast

    • Since 1980s

    • Greatly improved management of type 1 Diabetes


Synthesis of human insulin using recombinant dna technology
Synthesis of Human Insulin Using Recombinant DNA Technology

  • All involve

    • putting a human gene into a plasmid (which can either be a yeast or bacteria)

    • secreting a protein which insulin can be derived from


Step 1
STEP 1

  • Identify and Synthesize a human gene

  • Insulin:

    • Small protein

    • 51 amino acids- A&B

    • polypeptide chains

    • STEP: Chemically synthesize DNA chains that have nucleotide sequences for A and B “genes”


Step 2
STEP 2

  • Insert the Synthetic DNA into Plasmids

  • A and B nucleotide sequences individually put into gene for bacterial enzyme

  • Makes bacteria produce insulin


Step 3
STEP 3

  • Put Plasmid in Bacterial Cell

  • Recombinant plasmids introduced to E. coli

  • Requires millions of copies of bacteria w/ plasmid of insulin gene

  • Insulin expressed when gene replicates with B-galactosidase

  • Cell undergoes mitosis


Step 4
STEP 4

  • Make functional protein

  • A and B chains extracted from B-galactosidase and purified

  • Mixed together

  • Reconnected in disulfide cross

  • Then made suitable for injection


Other human proteins made with genetic engineering
Other Human Proteins Made with Genetic Engineering

  • Erythropoetin- treats anaemia in kidney failure patients

  • Human Growth Hormone- used to treat pituitary dwarfism

  • Insulin- Diabetes

  • Interferon- can treat Hepatitis B and C, some cancers, and MS

  • Factor VIII- treat hemophilia caused by lack of factor VIII


Factor viii ryan white
Factor VIII- Ryan White

  • Hemophiliac who got AIDS from a blood transfusion

    • Blood donation has risks - HIV and hepatitis

    • So, GM bacteria replaced blood donation for hemophiliacs

    • Factor VIII produced synthetically

      • Helps clot blood (factor)


Human growth hormone
Human Growth Hormone

  • Pituitary Dwarfism- treated by Human Growth Hormone

    • Before: extracted from pituitary glands of corpses

      • Some problems

        • Creutzfeldt-Jacob disease (CJD)

          • Brain disease

    • Now: GE bacteria

    • Injection


ad