Binding curve of C-reactive protein on HAECs

1. Binding curve of C-reactive protein on HAECs S. Devaraj et al. ATVB 2005;26:1359-1363

2. Inhibition of biotinylated CRP binding to HAECs S. Devaraj et al. ATVB 2005;26:1359-1363

3. Internalization of CRP S. Devaraj et al. ATVB 2005;26:1359-1363

4. Expression of Fcgamma receptors on HAECs S. Devaraj et al. ATVB 2005;26:1359-1363

5. Visualization of B-CRP, CD32, and CD64 by confocal microscopy A) Cells were incubated for 60 minutes at 4°C for maximal binding along with CRP (B-CRP followed by streptavidin-FITC), and expression of CD32 (PE) and CD64 (PE) were assessed. B and C, Intracellular localization of CRP (FITC), CD32 (PE, b), and CD64 (PE, c) after internalization with CRP (100 g/mL) for 120 minutes at 37°C by fluorescence microscopy. S. Devaraj et al. ATVB 2005;26:1359-1363

6. A and B, Effect of antibodies to Fcgamma receptors on CRP-induced IL-8 release (A) and CRP-induced inhibition of prostacyclin (B) S. Devaraj et al. ATVB 2005;26:1359-1363 Results are mean of 6 different experiments *P<0.001 compared with control, #P<0.05 compared with CRP

7. Effect of Antibodies to CD32 and CD64 on the Biological Effects of CRP in HAEC S. Devaraj et al. ATVB 2005;26:1359-1363