1 / 9

Final Meeting Ljubljana – 18.02.2013

Summary of ACW work in Q-Detect. Andreas Bühlmann JF Pothier, THM Smits, JE Frey, B Duffy (ACW, Switzerland). Final Meeting Ljubljana – 18.02.2013. www.qdetect.org. XAP LAMP assay. Xanthomonas arboricola pv. pruni Bacterial spot of stone fruit ( Prunus ). Assay designed Validated

huela
Download Presentation

Final Meeting Ljubljana – 18.02.2013

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Summary of ACW work in Q-Detect Andreas Bühlmann JF Pothier, THM Smits, JE Frey, B Duffy (ACW, Switzerland) Final Meeting Ljubljana – 18.02.2013 www.qdetect.org

  2. XAP LAMP assay Xanthomonas arboricola pv. pruni Bacterial spot of stone fruit (Prunus) • Assay designed • Validated • Ring tested • Paper published • EPPO validation Final Meeting Ljubljana – 18.02.2013

  3. Ea LAMP assay Erwinia amylovora Fire blight of pome fruit (Spiraeoideae) • Assay designed • Validated • Field tested (only CH) • Paper published • EPPO validation • 96 full genomes confirm presence of diagnostic target Final Meeting Ljubljana – 18.02.2013

  4. Further assays for plant pathogenic bacteria • Comparative genomics, primer design, validation with partners on… • Pantoea ananatis (ready for validation) • Pseudomonas syringae pv. actinida (genome sequenced, assay ready) • Xanthomonas oryzae pv. oryzae (Valerie Vérdier, F/USA) • Xanthomonas oryzae pv. oryzicola Final Meeting Ljubljana – 18.02.2013

  5. Insect LAMP assays developed • Mostly on COI • Thrips palmi • Bemisia tabaci • Scirtothrips dorsalis • Bactrocera dorsalis group Final Meeting Ljubljana – 18.02.2013

  6. Multiplexing LAMP • Swiss quarantine inspection service is considering Genies… • Would need: • Thrips palmi  • Bactrocera spp.  • Bemisisa tabaci () • Liriomyza trifolii/sativae (>80% of samples) • Multiplexable by melt curve: • Single tube assays make • inspectors lifes easier • Works with single insects Ea 86.0°C Bt 83.3°C Tp 81.8°C However - Multicolor still not done Final Meeting Ljubljana – 18.02.2013

  7. Airport diagnostics CH • LAMP canimproveimportdiagnostics • Bemisiatabaciassayoptimized Final Meeting Ljubljana – 18.02.2013

  8. SNP typing with LAMP • LAMP is not specific enough to detect SNPs • Same as TaqMan • LNA does not increase specificity but Speed. Final Meeting Ljubljana – 18.02.2013

More Related