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Badre & Cooper. Reduced Calcium Channel Function in the Drosophila cac TS2 mutant on Vision, Olfaction and Regulation of the Heart. Univ of Kentucky, Department of Biology. Previous Work. What are the Cac mutants?. Drosophila mutants:

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Badre cooper

Badre & Cooper

Reduced Calcium Channel Function in the DrosophilacacTS2 mutant on Vision, Olfaction and Regulation of the Heart

Univ of Kentucky, Department of Biology


Previous work
Previous Work

  • What are the Cac mutants?

  • Drosophila mutants:

    • The mutated gene cacTS2, is a temperature sensitive paralytic allele of Drosophila calcium channel subunit gene (Brooks et al., 2003)

  • Known Effects:

    • Decrease synaptic transmission at the NMJ (Kawasaki et al., 2004).

    • Role in male courtship behavior (Chan et al., 2002)

    • Role in nerve development (Xing et al. 2005).


Learning test larvae
Learning Test - Larvae

Vision - Learning Part

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Light + FRU

Dark + QUI

Gerber et al., 2003

Olfaction - Learning Part

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OCT + FRU

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Hendel et al., 2005


Results learning vision 33 c
Results Learning Vision - 33°C

  • 24 animals

  • 4 trials


Results learning olfaction 33 c
Results Learning Olfaction - 33°C

  • 40 animals

  • 2 trials


T maze adults

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T-maze - Adults

9.5 cm

9.5 cm

20.1 cm

1.2 cm

2.3 cm

2.3 cm

2.3 cm

2.3 cm



Results t maze vision 1 33 c

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Results – T-maze Vision 1 – 33°C

n = 5







Summary
Summary

  • Cac mutants larvae have decreased abilities to learn in both vision and olfaction at higher temperatures.

  • Cac mutants adults have decreased perception in both vision and olfaction at higher temperatures.

  • Cac mutants larvae do not have significantly altered heart rate at higher temperature.


Future directions
Future Directions

  • I am currently working on electroretinograms and electro-olfactorygrams .

Acknowledgments: Arnold and Mabel Beckman Foundation, Ribble Fellowship, University of Kentucky Undergraduate Research Program, Society for the Promotion of Undergraduate Research.


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