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Natural Solutions - Mealybug (Planococcus citri) Product #2 - Inbiol (5%) + Verticilium (95%)

Natural Solutions - Mealybug (Planococcus citri) Product #2 - Inbiol (5%) + Verticilium (95%) Dr. Michael P. Parrella & Thomas P. Costamagna. - Natural Solutions - Product #2 - Inbiol (5%) + Verticilium (95%). Objective To document efficacy and phytotoxcity. Target

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Natural Solutions - Mealybug (Planococcus citri) Product #2 - Inbiol (5%) + Verticilium (95%)

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  1. Natural Solutions - Mealybug (Planococcus citri) Product #2 - Inbiol (5%) + Verticilium (95%) Dr. Michael P. Parrella & Thomas P. Costamagna

  2. - Natural Solutions - Product #2 - Inbiol (5%) + Verticilium (95%) • Objective • To document efficacy and phytotoxcity. • Target • Citrus mealybugs (Planococcus citri) • All stage eggs, crawlers, adults. • Evaluation • Photos will be taken before and after to determine efficacy of materials being applied

  3. UC Davis - Greenhouse Trial - Crop Information - • Rose - Hydroponic - Cultivar ‘Avalanche’ • Plant Date - Week 26 - 2008 • Planting Total = 54 - 3 Blocks of 18 Plants • Media: Coco Coir Custom Blend + Bokashi • Daily Average Temperature 70 (HI 75 LO 65) • 16 Hours of Light - 12 Natural 4 Artificial • Vaporize Sulfur 1-4am - 3hrs

  4. Experiment Site - Rotating Phytotron

  5. Population Evaluation & Tagging • Prior to assigning treatments to blocks mealybug populations was assessed for distribution and density. • Presence of all stages was determined : egg, crawlers and adults. • Using a white tag marked with the Block # (I, II, or III) • 4 to 5 stems per plant were identified and appropriately marked for evaluation. • Pictures is the media being utilized for evaluation, comparing before to after.

  6. Block I - Before

  7. Block II - Before

  8. Before Block - III

  9. - Treatments - • Treatment A - Silwet 77 - 0.3% Solution • Treatment B - Natural Solutions 1:1000 + Silwet 77 - 0.3% Solution • Treatment C - UTC - Untreated Control • Random number generator used to assign treatments to block. http://www.random.org/ • Block I = A, Block II = B, Block III=C

  10. Application Details • 20 Liters of material mixed and applied to each Block. • Block = 18 plants = 1.11 Liter per Plant • Material applied before dawn < 70oF to maximize contact time and reduce possibility of phytotoxcity. • Single application with 24 hour post evaluation.

  11. After Block I - Silwet 77

  12. After - Block IINatural Solutions + Silwett 77

  13. After - Block III - UTC

  14. Treatment A - Silwet 77 - 0.3% Solution After Before Indicated by red arrows above one can see that Treatment A did not affect adult mealybug.

  15. Treatment A - Silwet 77 - 0.3% Solution After Before Indicated by red arrows above one can see that Treatment A had a negligible effect on mealybug egg masses.

  16. Treatment B - Natural Solutions 1:1000 + Silwet 77 After Before Indicated by red arrows above one can see that Treatment B caused mortality to adult mealybug.

  17. Treatment B - Natural Solutions 1:1000 + Silwet 77 After Before Indicated by red arrows above one can see that Treatment B penetrated mealybug egg masses and caused mortality.

  18. Treatment C - Untreated Control After Before Indicated by red arrows above one can see that Treatment C did not affect adult mealybug.

  19. Treatment C - Untreated Control After Before Indicated by red arrows above one can see that Treatment C had a negligible effect on mealybug egg masses.

  20. Conclusion Verticillium is a well-known entomopathogenic fungus which has been commercialized for aphid and whitefly control (Milner, 1997). The use of Verticillium for mealybug control is an uncommon practice and not well documented. This unique formulation of Product #2 by Natural Solutions + Silwett 77 (0.3% Solution) showed exceptional efficacy against all stages mealybugs in this trial with a single application. This material showed no phytotoxcity and should be further tested. The rapid mortality seen in this trial, 24 hours post spray, is not often associated with fungus used for pest control and should be studied to better understand its potential and role in pest management program.

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