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Mouse cage change criteria. Goals: Provide healthy environment Reduce animal stress Minimize care costs Minimize odors. Special Considerations. Microisolator cage tops hold in waste gases and moisture Small amounts of bedding dry out- excessively deep bedding stays damp

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mouse cage change criteria

Mouse cage change criteria

Goals:

Provide healthy environment

Reduce animal stress

Minimize care costs

Minimize odors

special considerations
Special Considerations
  • Microisolator cage tops hold in waste gases and moisture
  • Small amounts of bedding dry out- excessively deep bedding stays damp
  • Moisture from the water bottle is as bad as moisture from urine
more biology than you expected
More biology than you expected
  • Ammonia (NH3) is the gas of most concern- it irritates eyes, damages lungs.
  • Urine, NOT feces, is the source of ammonia
  • FRESH urine contains no ammonia
  • Over time, bacteria convert the urea in urine to ammonia
  • The urine needs to be wet for this to happen
mouse behavior
Mouse behavior
  • How do mice feel about a new, clean cage?
mouse poop is not the problem
Mouse poop is not the problem
  • Mice like feces
  • It is normal for mice to EAT feces
urine is the problem
Urine is the problem
  • Wet urine is the problem
  • Water (from the water bottle) is also a problem if it rehydrates dried urine
when do you need to change the cage
When do you need to change the cage?
  • When more than 25% of the bedding, as viewed from the bottom, is wet
  • When the bedding, as viewed from the top, is wet (don’t count a fresh drip from bottle)
  • If you see any mold on the bedding or food
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