Mouse cage change criteria
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Mouse cage change criteria. Goals: Provide healthy environment Reduce animal stress Minimize care costs Minimize odors. Special Considerations. Microisolator cage tops hold in waste gases and moisture Small amounts of bedding dry out- excessively deep bedding stays damp

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Mouse cage change criteria

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Mouse cage change criteria

Mouse cage change criteria

Goals:

Provide healthy environment

Reduce animal stress

Minimize care costs

Minimize odors


Special considerations

Special Considerations

  • Microisolator cage tops hold in waste gases and moisture

  • Small amounts of bedding dry out- excessively deep bedding stays damp

  • Moisture from the water bottle is as bad as moisture from urine


More biology than you expected

More biology than you expected

  • Ammonia (NH3) is the gas of most concern- it irritates eyes, damages lungs.

  • Urine, NOT feces, is the source of ammonia

  • FRESH urine contains no ammonia

  • Over time, bacteria convert the urea in urine to ammonia

  • The urine needs to be wet for this to happen


Mouse behavior

Mouse behavior

  • How do mice feel about a new, clean cage?


Mouse poop is not the problem

Mouse poop is not the problem

  • Mice like feces

  • It is normal for mice to EAT feces


Movie

Movie


Urine is the problem

Urine is the problem

  • Wet urine is the problem

  • Water (from the water bottle) is also a problem if it rehydrates dried urine


When do you need to change the cage

When do you need to change the cage?

  • When more than 25% of the bedding, as viewed from the bottom, is wet

  • When the bedding, as viewed from the top, is wet (don’t count a fresh drip from bottle)

  • If you see any mold on the bedding or food


Five mice in a cage

Five mice in a cage

3 days

7 days


M4 d7

M4 D7


M5 d5

M5 D5


M1 d10

M1 D10


M1 d12

M1 D12


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