Chapter 20 Reading Quiz. Genes from two different sources that are combined result in ____. Where are “sticky ends” found? What structures, naturally found in bacteria, cut up foreign DNA? What is the point of the genetic technology “PCR”?
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- bacterial plasmids & foreign DNA with gene of interest
2. Insertion of gene-source DNA into the vector
- plasmid DNA cut mix foreign DNA and plasmids add DNA ligase to catalyze new covalent bonds
- naked DNA added to bacterial culture, plasmid DNA taken up by transformation
- reproduced and cloned
5. Identification of cell clones carrying the gene of interest (indicators added)
2. Complementary DNA made in the lab from mRNA templates
Viral genomic RNA
- a map made by cutting the DNA of each chromosome into a number of identifiable fragments
- used as genetic markers for making linkage maps
- are a “genetic fingerprint”
- produces linkage maps without the need for large family pedigree analysis
13. Describe how recombinant DNA technology can have medical applications such as diagnosis of genetic disease, development of gene therapy, vaccine production, and development of pharmaceutical products.
- many social and ethical questions
- gene splicing applies to insulin and growth hormone (genetically engineered human insulin)
Animals bovine growth hormone (made by ecoli); enhances milk production and weight gain
cellulase (ecoli) hydrolyzes cellulose making the whole plant good for feed
Plants easier to engineer – can be made from a single cell
- helps with herbicide resists pathogens and insects
- enhanced food value
- moves on to governments and regulatory agencies which promote potential industrial, medical, and agricultural benefits but ensure that the new products are safe