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An Experimental Model of Human Absorptive Enterocytes (Caco-2) to Assess the Bioavailability of Various Metals

An Experimental Model of Human Absorptive Enterocytes (Caco-2) to Assess the Bioavailability of Various Metals. Jonas Tallkvist

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An Experimental Model of Human Absorptive Enterocytes (Caco-2) to Assess the Bioavailability of Various Metals

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  1. An Experimental Model of Human Absorptive Enterocytes (Caco-2) to Assess the Bioavailability of Various Metals Jonas Tallkvist Department of Biomedical Sciences and Veterinary Public Health, Division of Pathology, Pharmacology and Toxicology, Swedish University of Agricultural Sciences, Uppsala, Sweden MITF 071018

  2. Bioavailability ”The fraction of ingested metal that reaches the systemic circulation”

  3. Factors Influencing Bioavailability • Dose • Age • pH • Gastrointestinal contents • Nutritional status • Disease

  4. Metals Investigated in Caco-2 Cells V Mn Pb Zn Cr Ni Fe Cu Co Mg Cd Ca

  5. Routes of Metal Absorption Active Passive Mucosal/apical side Enterocytes Basement membrane Blood vessel Blood/basolateral side

  6. The Caco-2 Cell Model Apical chamber Caco-2 cells Basolateral chamber Permeable filter support

  7. Cadmium Absorption Zalups and Ahmad (2003) Toxcol Appl Pharm, 186, 3

  8. Correlation Between DMT1 Expression and Cd Transport in Caco-2 Cells Tallkvist et al., (2001) Tox Lett, 122, 171

  9. Summary The Caco-2 cell model provides a useful tool to assess the bioavailability of metals at various defined experimental conditions. The Caco-2 cell model offers the possibility to gain insight into molecular mechanisms implicated in the bioavailability of metals.

  10. Thank-You for Your Attention!

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