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최기영

Transcriptional Cross-Regulation of the Catechol and Protocatechuate Branches of the β - Ketoadipate Pathway Contribute to Carbon Source-Dependent Expression of the Acinetobacter sp. Strain ADP1 pobA Gene. 최기영. Intro. In ADP1, benzoate is consumed in preference to 4-hydroxybenzoate (4HB)

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최기영

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  1. Transcriptional Cross-Regulation of the Catechol and Protocatechuate Branches of the β-Ketoadipate Pathway Contribute to Carbon Source-Dependent Expression of the Acinetobacter sp. Strain ADP1 pobA Gene 최기영

  2. Intro • In ADP1, benzoate is consumed in preference to 4-hydroxybenzoate (4HB) • BenR helps repress the expression of pcaK, the gene encoding a 4HB permease • 4HB interacts with the PobR regulator to activate pobA expression • cis,cis-muconate (CCM), a metabolite of benzoate degradation, rather than benzoate itself, is the key inhibitor of 4HB catabolism when ADP1 grows on benzoate

  3. Materials and Methods • Bacterial strains and growth conditions • β-galactosidase assay • Gel retardation assay • Metabolite monitoring by HPLC

  4. Carbon source-dependent regulation of pobA expression

  5. Inhibition of pobA expression during growth on substrates of the β-ketoadipate pathway

  6. Identification of regulatory proteins that inhibit the ability of PobR to activate pobA expression

  7. Benzoate and 4HB catabolism in mutants lacking BenM and CatM • CatM is not essential for inhibition of 4HB consumption during growth on benzoate

  8. Discussion • The CatM and BenM transcriptional regulators contribute to cross-regulation of the catechol and protocatechuate branches of the β-ketoadipate pathway • Connections between pca and pobA gene expression • Overlapping functions of CatM and BenM • Carbon source-dependent gene expression is complex

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