XXII Congresso Nazionale della Società Chimica Italiana
This presentation is the property of its rightful owner.
Sponsored Links
1 / 5

D. Dell’Atti , M. Minunni, S. Tombelli, M. Mascini PowerPoint PPT Presentation


  • 38 Views
  • Uploaded on
  • Presentation posted in: General

XXII Congresso Nazionale della Società Chimica Italiana FIRENZE, 10-15 SETTEMBRE 2006. Development of a DNA-based piezoelectric biosensor for the simultaneous detection and genotyping of high risk Human Papilloma Virus strains. D. Dell’Atti , M. Minunni, S. Tombelli, M. Mascini

Download Presentation

D. Dell’Atti , M. Minunni, S. Tombelli, M. Mascini

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


D dell atti m minunni s tombelli m mascini

XXII Congresso Nazionale della Società Chimica Italiana

FIRENZE, 10-15 SETTEMBRE 2006

Development of a DNA-based piezoelectric biosensor for the simultaneous detection and genotyping of high risk Human Papilloma Virus strains

D. Dell’Atti, M. Minunni, S. Tombelli, M. Mascini

Università degli Studi di Firenze, Dipartimento di Chimica

A. Cavazzana, M. Zavaglia, G. Bevilacqua

Università degli Studi di Pisa, Dipartimento di Oncologia, dei trapianti

e delle nuove tecnologie in medicina


D dell atti m minunni s tombelli m mascini

DNA Based Biosensors

Synthesisof a DNA fragment (probe)

containing the sequence of interest

Immobilisationof the probe onto the solid support of the sensor

Extraction of the DNA from the real sample (blood, water, food) andamplificationof the sequence of interest

(thiol/dextran/streptavidin/biotinylatedprobe)

Denaturation of the amplified fragment to obtain a single-stranded DNA

Hybridisation of the obtained ssDNA with the immobilised probe

Changes in the physicochemical parameters of the layer formed on the transducer


D dell atti m minunni s tombelli m mascini

Instrumentation

  • 9.5 MHz AT-Cut quartz crystal (14mm)

  • Gold evaporated (42.6mm2 area) on both sides

  • Crystal Manufactoring (USA)

Methacrylate cell

QCMagic (ElbaTech, Marciana, Italy)


D dell atti m minunni s tombelli m mascini

11-mercaptoundecanol

dextran

gold

streptavidin

biotinylated probe

Immobilisationprotocol


D dell atti m minunni s tombelli m mascini

Biosensor signals

Probe immobilisation

A. Hybridisation buffer

Target ( 10 min)

C. Hybridisation buffer

D. Regenerating solution

HCl 1mM ( 2*30”)

Hybridisation cycles

A

D

A

Analytical datum

is ∆F (Fc-Fa)

B

C


  • Login