Poster ID: EPP005.
Detection of antibodies to Burkholderia pseudomallei in children in Siem Reap, Kingdom of Cambodia and presence of the organism in their environmentVanaporn Wuthiekanun1, Dr. Ngoun Pheaktra2, Hor Putchhat2, Sayan Lankla1, Sin Lina2, Bun Sen2, Sharon Peacock1, Nicholas Day11. Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Road, Bangkok, Thailand; 2. Angkor Hospital for Children (AHC), Siem Reap, Kingdom of Cambodia
Study design: prospective, cross-sectional study
Study site: Angkor Hospital for Children (AHC) in Siem Reap, Cambodia
Study sample: Serum from 968 unselected consecutive patients 0-16 years old who had blood drawn at AHC for routine work-up
Measure: Indirect hemagglutination assay (IHA) to detect presence and titer of antibodies to B. pseudomallei
Environmental sampling: 4 soil samples were collected from each of 10 different paddy fields around Siem Reap. Culture & sensitivities for B. pseudomallei were done on the soil samples.
The presence of antibodies to B. pseudomallei in the serum of children and isolation of B. pseudomallei from the environmental soil as shown by this study provide evidence that,despite the lack of reported indigenous cases, melioidosis is likely to occur in Cambodia. These results also show a need for diagnostic microbiology development and associated detection of melioidosis cases across rural southeast Asia.