Over expression of Acetyl- CoA carboxylase (ACC ) sub-unit accC in E.coli to enhance fatty acid ( triacyl glycerol) accumulation for Bio-fuel production”. “Fuel it up” Team members: Parul Sirohi Sanju Timilsina. Goal:.
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Over expression of Acetyl- CoA carboxylase (ACC) sub-unit accCin E.coli to enhance fatty acid (triacyl glycerol) accumulation for Bio-fuel production”
“Fuel it up”
To overexpressed accC gene in E.coli to increases Tri acyl glycerol (TAG) production and then our future goal will be to express the accC gene in any possible microorganisms ( Algae and bacteria) which might enhance lipid production in waste biomass.
Source: NMD courchesne et.al./ journal of biotechnology 141(2009)31-41
Source Organism: E. coli 0157:H7
Source: Biology department of University of Northern Iowa
Media: Luria Broth
Gene: Acetyl CoA carboxylase biotin carboxylase (accC)
Assembly #: NC_011353.1 Region: 4242644..4243993 bp:1350
Introns: none ( prokaryote)
Bio-brick Compatibility: Compatible
pSB1A3 is a high copy number plasmid carrying Ampicillin resistance
pSB1A3 is a bio-brick compatible plasmid that has prefix(E-X) and suffix(S-P) once in the whole sequence
Its is also compatible for the constitutive promoter family member
-Materials for TLC: Silica gel,
-Solvent mixture hexane/diethyl ether/acetic acid(17/3/0.2/v/v/v)
-CuSO4 reagent ( 20g CuSO4, 200ml methanol, 8ml H2SO4 and
-acetone/ toluene/ water( 91/30/8,v/v/v)