Biomedical research mentoring with dr eric brandt
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Understanding the contribution of traffic related pollution on asthma . Biomedical Research Mentoring with Dr. Eric Brandt. Reda Baig. T raffic related pollution does not cause asthma but it exacerbates asthma . Allergens. Diesel Exhaust Particle (DEP).

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Biomedical Research Mentoring with Dr. Eric Brandt

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Biomedical research mentoring with dr eric brandt

Understanding the contribution of traffic related pollution on asthma

Biomedical Research Mentoring with Dr. Eric Brandt

Reda Baig


Biomedical research mentoring with dr eric brandt

Traffic related pollution does not cause asthma but it exacerbates asthma

Allergens

Diesel Exhaust Particle (DEP)

Inflammation results in smaller airways

Immune cells

Mucus Clogging of the airways

ASTHMA

Contains anti-inflammatory steroids & bronchodilator


Biomedical research mentoring with dr eric brandt

Our goal is to understand how exposure to diesel exhaust particles (DEP) exacerbates house dust mite (HDM) induced allergic responses (asthma attacks)

Protocol

saline, DEP, HDM or HDM+DEP

Day 1 4 6 8 11 13 15 18 20 24H

Lung cells

Centrifuge on glass slide

Assess the different cell types


Exposure to allergen hdm eosinophilic inflammation exposure to dep neutrophilic inflammation

Exposure to allergen (HDM) = eosinophilic inflammationExposure to DEP = neutrophilic inflammation

Saline

HDM

eosinophils

neutrophil

HDM+DEP

DEP

neutrophil


Biomedical research mentoring with dr eric brandt

Protocol

House

dust mite

DEP

saline, DEP, HDM or HDM+DEP

Day 1 4 6 8 11 13 15 18 20 24H

Lung tissue

RNA

cDNA

qPCR

LUNG EPITHELIAL CELLS

chemokines

Eotaxin-1

CCR3

RESULT

**

Fold increase

*

BLOOD VESSEL

Neutrophils

Eosinophils


What i ve learned

What I’ve Learned

  • Writing in a lab notebook

    • Every detail is important

  • Lab techniques

    • Identify and count cells from the airways

    • Isolate RNA from lung  reverse transcribe into cDNA

    • Math: Dilutions

    • Run qPCR and generate graphs of results

    • Run PCR products on agarose gels

  • Basic Immunology


Future

Future

  • Continue with research during the summer and throughout undergrad


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