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Understanding the contribution of traffic related pollution on asthma . Biomedical Research Mentoring with Dr. Eric Brandt. Reda Baig. T raffic related pollution does not cause asthma but it exacerbates asthma . Allergens. Diesel Exhaust Particle (DEP).

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Presentation Transcript
slide2

Traffic related pollution does not cause asthma but it exacerbates asthma

Allergens

Diesel Exhaust Particle (DEP)

Inflammation results in smaller airways

Immune cells

Mucus Clogging of the airways

ASTHMA

Contains anti-inflammatory steroids & bronchodilator

slide3

Our goal is to understand how exposure to diesel exhaust particles (DEP) exacerbates house dust mite (HDM) induced allergic responses (asthma attacks)

Protocol

saline, DEP, HDM or HDM+DEP

Day 1 4 6 8 11 13 15 18 20 24H

Lung cells

Centrifuge on glass slide

Assess the different cell types

exposure to allergen hdm eosinophilic inflammation exposure to dep neutrophilic inflammation
Exposure to allergen (HDM) = eosinophilic inflammationExposure to DEP = neutrophilic inflammation

Saline

HDM

eosinophils

neutrophil

HDM+DEP

DEP

neutrophil

slide5

Protocol

House

dust mite

DEP

saline, DEP, HDM or HDM+DEP

Day 1 4 6 8 11 13 15 18 20 24H

Lung tissue

RNA

cDNA

qPCR

LUNG EPITHELIAL CELLS

chemokines

Eotaxin-1

CCR3

RESULT

**

Fold increase

*

BLOOD VESSEL

Neutrophils

Eosinophils

what i ve learned
What I’ve Learned
  • Writing in a lab notebook
    • Every detail is important
  • Lab techniques
    • Identify and count cells from the airways
    • Isolate RNA from lung  reverse transcribe into cDNA
    • Math: Dilutions
    • Run qPCR and generate graphs of results
    • Run PCR products on agarose gels
  • Basic Immunology
future
Future
  • Continue with research during the summer and throughout undergrad
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