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National Biology. Proteins and enzymes e: Effect of temperature and pH. Recap. Entrance ticket or whiteboard Explain how you would use Benedict’s solution to test for a reducing sugar. What are we learning today?. We are learning to:

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National Biology

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National biology

National Biology

Proteins and enzymes

e: Effect of temperature and pH


Recap

Recap

  • Entrance ticket or whiteboard

  • Explain how you would use Benedict’s solution to test for a reducing sugar


What are we learning today

What are we learning today?

We are learning to:

  • Explain the effect of temperature on enzyme activity in terms of the shape of the active site

  • State what is meant by term ‘optimum’

  • State what is meant by the term ‘denatured’


Experiment

Experiment

Aim: to find out the effect of temperature on activity of enzymes

D 5mls hydrogen peroxide 80oC water bath

C 5mls hydrogen peroxide 40oC water bath

A 5mls hydrogen peroxide ice bath

B 5mls hydrogen peroxide 20oC room temp


Conclusion

Conclusion

  • As temperature increases the activity of the enzyme increases up until temperatures around 40oC, after which the activity decreases.


Explanation

Explanation

  • When enzymes are heated up they change shape:

  • When enzymes change shape we say that they have been DENATURED

  • This means that they will not work because the substrate will no longer fit into the active site.

Enzyme working

Denatured enzyme

Not working

enzyme

enzyme


What is the optimum

What is the optimum?

The optimum temperature is the temperature at which an enzyme is the most active


What effect does ph have on enzyme activity

What effect does pH have on enzyme activity?

  • Enzymes have an optimum pH

  • Enzymes have a narrow pH range at which they function


Effect of ph on enzyme activity

Effect of pH on enzyme activity

Rate of

reaction

Salivary amylase will not work at pH 1 and pepsin will not work at pH 9


Summary

Summary

  • What does the word optimum mean?

  • What does the word denatured mean?


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