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6 .1. Основни етапи в генното клониране - PowerPoint PPT Presentation


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6 .1. Основни етапи в генното клониране. 6 .2. Плазмидите като вектори. 1; лесно да се пренасят в акцепторната клетка. 2; да се намножават в голямо количество. 6.3. Конструиране на плазмиден вектор. 6.4. Амплификация на ДНК фрагмент чрез клониране в плазмиден вектор. λ - фаг. фаг M13.

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6 .1. Основни етапи в генното клониране

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6.1.


6.2. . 1; . 2;


6.3.


6.4.


-

M13

6.5.


6.6.


6.7. E. coli . .


6.8.


6.9. 13


6.10. EcoRI.


6.13. , .


6.14. -


6.15. -. , - . - ,


6.16. (dNTP) - (ddNTP). ddNTP .


6.17. Sanger


6.18.


6.19. 15

. : ) BamHI; b) EcoRI; c) BamHI + EcoRI; d)PstI; e)


6.20.


6.21. RFLP .


6.22.


6.23. 3 (1-3). (-).


6.24. .


6.25.


6.26. , PCR


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