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6 .1. Основни етапи в генното клониране PowerPoint PPT Presentation


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6 .1. Основни етапи в генното клониране. 6 .2. Плазмидите като вектори. 1; лесно да се пренасят в акцепторната клетка. 2; да се намножават в голямо количество. 6.3. Конструиране на плазмиден вектор. 6.4. Амплификация на ДНК фрагмент чрез клониране в плазмиден вектор. λ - фаг. фаг M13.

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6 .1. Основни етапи в генното клониране

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6.1.


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6.2. . 1; . 2;


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6.3.


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6.4.


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-

M13

6.5.


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6.6.


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6.7. E. coli . .


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6.8.


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6.9. 13


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6.10. EcoRI.


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6.13. , .


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6.14. -


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6.15. -. , - . - ,


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6.16. (dNTP) - (ddNTP). ddNTP .


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6.17. Sanger


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6.18.


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6.19. 15

. : ) BamHI; b) EcoRI; c) BamHI + EcoRI; d)PstI; e)


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6.20.


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6.21. RFLP .


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6.22.


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6.23. 3 (1-3). (-).


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6.24. .


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6.25.


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6.26. , PCR


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