Human cells stained with an antibody for the Tubulin protein
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Human cells stained with an antibody for the Tubulin protein Image from Molecular Probes. GFP-RAS2 in living Saccharomyces cerevisiae. Images from Roger Tsein’s Lab. Making a Fusion Protein:. Yak1 Gene: DNA: AAAG ATG AATCAG ... ACATCG TGA CCTA... Protein: N- Met-Asn-Gln-…-Thr-Ser-C

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Human cells stained with an antibody for the Tubulin protein Image from Molecular Probes

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Human cells stained with an antibody for the Tubulin protein

Image from Molecular Probes


GFP-RAS2 in living Saccharomyces cerevisiae


Images from Roger Tsein’s Lab


Making a Fusion Protein:

Yak1 Gene:

DNA: AAAGATGAATCAG ... ACATCGTGACCTA...

Protein: N-Met-Asn-Gln-…-Thr-Ser-C

GFP Gene:

DNA: GCAAATGAGTAAA ... CTGAAATAAGTAC...

Protein: N-Met-Ser-Lys-… -Leu-Lys-C

Yak1

GFP


Making a Fusion Protein:

  • Yak1 Gene:

  • DNA: AAAGATGAATCAG ... ACATCGTGACCTA...

  • GFP Gene:

  • DNA: GCAAATGAGTAAA ... CTGAAATAAGTAC...

  • Fusion Gene:

  • AAAGATGAATCAG ... ACATCGATGAGTAAA ... CTGAAATAATGACCTA...


  • Making a Fusion Protein:

    Yak1 Gene:

    DNA: AAAGATGAATCAG ... ACATCGTGACCTA...

    Protein: N-Met-Asn-Gln-…-Thr-Ser-C

    GFP Gene:

    DNA: GCAAATGAGTAAA ... CTGAAATAAGTAC...

    Protein: N-Met-Ser-Lys-… -Leu-Lys-C

    Yak1

    GFP

    N

    C

    Fusion Gene:

    AAAGATGAATCAG ... ACATCGATGAGTAAA ... CTGAAATAATGACCTA...

    Fusion Protein:

    N-Met-Asn-Gln-…-Thr-Ser-Met-Ser-Lys-… -Leu-Lys-C


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