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نبذة تاريخية عن أمراض النبات البكتيرية PowerPoint PPT Presentation


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نبذة تاريخية عن أمراض النبات البكتيرية.

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نبذة تاريخية عن أمراض النبات البكتيرية

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2763181

  • 1878 Thomas Burrill Illinois . . .


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  • Micrococcus amylovorus Erwinia amylovora . . . . 1920 E. amylovora .


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1-

  • ProKaryotae .

. .

  • . . .


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1-1

Streptomyces . 0.6-3.5 0.5 1 . X Y . .

  • Slime Layer Capsule .


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Clockwise from above, scanning EM, Streptomyces, capsule


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  • Nitomycine Acridine Orange (35 40 ) Agrobaoterium tumefaciens Octopine & Nopaline .

  • Plasmids Cryptic Plasmids .


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  • Plasmids Episome .

  • . (-2).

  • Streptomyces .


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  • Envelope Polysaccharides Lipopolysaccharides .

  • Plasmids DNA Covalent - 200 kb Sex Factors UV .


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  • .

2


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3

(A) (B)


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  • . . . 1 . . .. . ... . . .

  • . . .


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  • Single cell, prokaryotic, no organelles, some are motile

  • 0.5-1 X 1-10 m

  • Rod, coccoid, spiral, pleomorphic shaped

  • Chemoheterotrophs (some are fastidious)

  • Assexual, transverse binary fission


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1-2

  • Mesosome . . .


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4


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  • . .

  • . :


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1 :Lag phase

.

2 :Log phase

. .

3 : Stationary phase

.


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4 :Death or Decline phase

  • . Phase of Logarithmic Death .

5


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1-3

  • . Erwinia amylovora . . Agrobacterium tumefaciens . Erwinia Pseudomonas .


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  • . .... .

  • Epiphytically . .

  • .


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1-4

  • . . . . .


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1-5

  • Streptomyces . . . pH . .


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  • .

  • .

  • . .


Gram positive classification

Negative

Gram Reaction

Positive

+

-

Endospores

+

-

+

-

Anerobic

Aerial mycelia

Clostridium:smelly rots

Bacillus: scald,

palm

Streptomyces:

scab

Coryneform: leaf and stem

Spots and necrosis, wilts

Gram Positive Classification


Gram negative classification

Negative

Gram Reaction

Positive

+

-

Anaerobic

Yellow

colonies

Fluorescent

+

-

+

-

Pantoea: wilts,

necrosis

Erwinia: necrosis,

Soft rots

Yellow

colonies

+

-

Gram Negative Classification

Pseudomonas:

Necrosis, chlorosis,

Gummosis, galls, etc.


Gram negative non fluorescent bacteria

Yellow

colonies

+

-

Xanthomonas: necrosis,vascular discoloration,chlorosis

Growth on D1M

+

-

Betaine and

Arginine use

+

-

or

Xylophilus:blight

Growth at 40 C

+

-

Gram negative, non-fluorescent bacteria

Agrobacterium:galls, wilts, dieback,hairy root

Burkholderia:necrosis, wilt, rot

Acidovorax:blights, spots,blotches

Ralstonia:wilts


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  • ( ) (1 ). tumefaciensAgrobacterium Over Growth (Tumor) Proliferation Pseudomonas, Gorynebacteriam.


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  • . .

  • Antisera . .


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  • Bacteriocins . . Plasmid-DNA .

  • . .


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(1) :


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6


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7


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