Supplementary Fig
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Kda

Supplementary Fig. 1. Expression and purification of Bacillus sp. BRC1 Bdh in/from E. coli. Lane 1, total cell lysates of E. coli BL21 harboring pET28a (control); lane 2, total cell lysates of E. coli BL21 harboring pET-bhd; lane 3, soluble fraction; lane 4, insoluble fraction; lane 5, purified Bdh (arrow).

M

1

2

3

4

5

kDa

130

95

55

36

28

Supplementary Fig. 1


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Supplementary Fig. 2.Effects of pH (A) and temperature (B) on Bdhactivity toward the substrates D-2,3-BD (closed circles) and acetoin (open circles).

A

B

Supplementary Fig. 2


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Supplementary Fig. 3. Enzyme kinetics of Bacillus sp. BRC1 Bdh. A, D-2,3-BD; B, Meso-2,3-BD; C, Acetoin; D, Diacetyl

A

B

C

D

Supplementary Fig. 3


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Supplementary Fig. 4. Enzyme activity of Bdh from recombinant Bacillus sp. BRC1 strains. Closed bars, D-2,3-BD; open bars, acetoin.

BRC1/pWH

BRC1/pWH-bdh

Xylose 5%

Xylose 5%

Xylose 20%

Xylose 20%

EFB hydrolysate

EFB hydrolysate

Supplementary Fig. 4


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