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Anatomy of a Gene

Anatomy of a Gene. Bellwork. Objective: SWBAT describe and draw the anatomy of a gene as well as the function of each piece. Bellwork Question: Please transcribe and translate this DNA strand T G C G A T T A C G G G A C C A T T A G C C G T. Anatomy of a gene.

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Anatomy of a Gene

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  1. Anatomy of a Gene

  2. Bellwork • Objective: SWBAT describe and draw the anatomy of a gene as well as the function of each piece. • Bellwork Question: • Please transcribe and translate this DNA strand • T G C G A T T A C G G G A C C A T T A G C C G T

  3. Anatomy of a gene • Genes have much more to them than just A-T-G-C. • Some of the questions you have asked lead into this topic: • Why are there more bases before and after what is actually translated?

  4. Parts of a Gene: • 5’ Cap- used to signify that the 5’ end is complete and ready to leave the nucleus. • 5’ and 3’ un-translated region (UTR)- Used as buffers to protect the coding sequence. • Coding sequence- Codes for the protein. Composed of introns and exons. • Poly-A tail- Used to signify that the mRNA is complete and ready to leave the nucleus.

  5. Introns and Exons • Introns and Exons are coded for in the DNA. • Exons code for proteins. Introns get spliced out. • (Video http://www.youtube.com/watch?v=2zBBm2E0aZ4 )

  6. Talk to your neighbor and answer in your notes. • WHY might introns be important even if they don’t get translated into a protein?

  7. There is no one way to splice a gene! • Allows one gene to code for many different proteins.

  8. Let’s Practice • Translate this: Green are introns. Blue are Exons. • CAP-5’ UTR- GAC-UAU-AUG-AAA-CAG-GCG-AGC-UGA-UAA-3’UTR-AAAA

  9. Check for Understanding • Why is it important to have genetic material, both contained in the coding region and outside the coding region, that doesn’t get translated into amino acids? • Answer this in your notes.

  10. What happens when mutations occur? • You have mutations all throughout your genome. • Few are deleterious, many are unseen. • What happens when you have a mutation that messes up intron splicing?

  11. On a separate sheet of paper-you will turn this in!!! • Translate this: Green are introns. Blue are Exons. • CAP-5’ UTR- GAC-CUU-AUG-AAA-CAG-AAA-AGC-UAA-3’UTR-AAAA • What is the protein you end up with? • Why are the CAP and poly A tail important? • Why are the UTRs important? • Why are the Introns and exons important?

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