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# AP Bio Lab 1 - PowerPoint PPT Presentation

AP Bio Lab 1. Water Potential. The combined effect of: Solute concentration Pressure …are incorporated into a single measurement called water potential Water moves from the area of HIGH water potential  to the area of LOW water potential when moving across a membrane.

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Presentation Transcript

### AP Bio Lab 1

• The combined effect of:

• Solute concentration

• Pressure

…are incorporated into a single measurement called water potential

• Water moves from the area of HIGH water potential  to the area of LOW water potential when moving across a membrane

Solute Potential pressure*

• Pure water has a solute potential of zero. Solute potential is NEVER positive.

• Adding more solute is a negative experience; the solute potential always becomes negative or more negative

Sample Problem

• The molar concentration of a sugar solution in an open beaker has been determined to be 0.3M. Calculate the solute potential at 27 degrees. Round your answer to the nearest hundredth.

• If the sugar solution was placed inside of dialysis tubing and put in a beaker of pure water (Ψ=1) what would happen? Why?

Pressure Potential pressure*

• Is the sum of all pressure on water

• Get b/c of 2 things:

• Turgor pressure- force created by the cell membrane pushing against the cell wall (must have water inside to push cell wall out)

• You CAN’T know this unless given so assume that the pressure potential is zero unless otherwise told

QUIZ TIME!!!! pressure*

Lab Set Up pressure*

• Changes to procedure:

• Part B, bags are 15cm long NOT 30cm

• Part B, add 10ml of each sucrose solution to each bag

• You don’t know the molarities they are labeled (A-F). You must determine which solution goes to which molarity based on your collected data.

• Put all dialysis bags and string in the same beaker for the weekend and cover!

• No cork borer for potato cylinders so cut 4 relatively equally sized cubes for each solution  REMOVE ALL SKIN (set up scales first before getting so you can mass and drop in the solutions immediately).

• Label all beakers that are using/will use