Ap bio lab 1
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AP Bio Lab 1. Water Potential. The combined effect of: Solute concentration Pressure …are incorporated into a single measurement called water potential Water moves from the area of HIGH water potential  to the area of LOW water potential when moving across a membrane.

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AP Bio Lab 1

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Ap bio lab 1

AP Bio Lab 1


Water potential

Water Potential

  • The combined effect of:

    • Solute concentration

    • Pressure

      …are incorporated into a single measurement called water potential

  • Water moves from the area of HIGH water potential  to the area of LOW water potential when moving across a membrane


H20 moves from areas of high pressure to areas of low pressure

*H20 moves from areas of high pressure to areas of low pressure*


Solute potential

Solute Potential

  • Pure water has a solute potential of zero. Solute potential is NEVER positive.

  • Adding more solute is a negative experience; the solute potential always becomes negative or more negative

    Sample Problem

  • The molar concentration of a sugar solution in an open beaker has been determined to be 0.3M. Calculate the solute potential at 27 degrees. Round your answer to the nearest hundredth.

  • If the sugar solution was placed inside of dialysis tubing and put in a beaker of pure water (Ψ=1) what would happen? Why?


Pressure potential

Pressure Potential

  • Is the sum of all pressure on water

  • Get b/c of 2 things:

    • Turgor pressure- force created by the cell membrane pushing against the cell wall (must have water inside to push cell wall out)

  • You CAN’T know this unless given so assume that the pressure potential is zero unless otherwise told


Ap bio lab 1

QUIZ TIME!!!!


Lab set up

Lab Set Up

  • Changes to procedure:

    • Part B, bags are 15cm long NOT 30cm

    • Part B, add 10ml of each sucrose solution to each bag

    • You don’t know the molarities they are labeled (A-F). You must determine which solution goes to which molarity based on your collected data.

  • Put all dialysis bags and string in the same beaker for the weekend and cover!

  • No cork borer for potato cylinders so cut 4 relatively equally sized cubes for each solution  REMOVE ALL SKIN (set up scales first before getting so you can mass and drop in the solutions immediately).

  • Label all beakers that are using/will use


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