1 / 10

Chapter 14 RNA Processing I : Splicing

Chapter 14 RNA Processing I : Splicing. Summary of splicing Transcription Primary transcript  splicing mature transcript. Evidences for introns R-loop in the electron microsopic view of chromatin sequencing ; compare genomic DNA and cDNA. Splicing signals and mechanism.

harley
Download Presentation

Chapter 14 RNA Processing I : Splicing

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Chapter 14 RNA Processing I : Splicing

  2. Summary of splicing • Transcription • Primary transcript •  splicing • mature transcript • Evidencesfor introns • R-loop in the electron microsopic view of chromatin • sequencing ; compare genomic DNA and cDNA

  3. Splicing signals and mechanism Lariat structure Introns almost all start with GU and end with AG Branch point Spliceosome ; pre-mRNA, snRNPs (snurps); protein RNA ; U1,2,4,5,6

  4. Spliceosomal pre-mRNA U6 U2 U5

  5. The spliceosome cycle Model for participation of CTD in exon definition - The CTD acts as a platform for the gathering of pre mRNA and splicing factors.

  6. Alternative splicing Ex) Mu RNA; secreted form and membrane-bound form Sex determination in Drosophila ; 8 chromosomes (X/Y, 2, 3, 4 autosomes) male = XY female= XX Sxl activates tra activates dsx to determine female.

  7. serendipity Self-splicing RNAs Group I intron Group II intron Thomas Cech ; Nobel prize 1989 Any possibility of contamination of spliceosome in the in vitro transcription?  chemically synthesize the precursor  splicing still occurs Tetrahymena (ciliate)

  8. Group I intron GTP

  9. Spliceosomal pre-mRNA Group II intron U6 Group II intron’s splicing - - - mechanism is similar to pre mRNA’s splicing - They have A at the branch point. U2 U5

  10. Is self-splicing RNA true enzyme? Phe • CatalyticRNA = Ribozyme • self-splicing RNA • M1 RNA of RNase PI • Telomerase • CoTc 5’ RNaseP

More Related