Extraction of Human DNA. Experiment Goals. Isolation of genomic DNA from human blood Analysis of isolated DNA using Agarose gel electrophoresis Spectrophotometry. What is a DNA?. DNA, also known as deoxyribonucleic acid, A fundamental molecule found in all living things
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DNA sample by the
volume of hydrating solution added.
Example for DNA: 150 µg/mL X 0.1 mL = 15 µg
Concentration from UV Spec. (µg DNA per ml of hydrating solution)
Volume of hydration solution
DNA yieldQuantity from UV Spectrophotometry Calculating Yield