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Център по молекулна медицина PowerPoint PPT Presentation


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Лазерната микродисекция- безценният инструмент на молекулярната патология и диагностика, съдебната медицина и съвременната наука. Център по молекулна медицина. Нова биомедицинска революция.

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Център по молекулна медицина

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  • LM ( )

    • Lowry and Passonneau-

    • Goelz et al-


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,


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(LM)

  • Meier-Ruge et al- 1976, LM UV , , -

  • Shibata- 1993-UV , (Selective ablation of unwanted regions)

  • Emmert-Buck et al- 1996 NIH- Arcturus Engineering


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LM

  • /


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LCM


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LM

  • IR LCM- EVA , .

  • UV LM- ,

  • IR UV- Arcturus Veritas Acturus XT


Ir lcm

IR-LCM


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LCM

LCM

LCM


Ir lcm1

IR LCM

  • Arcturus/MDS, Inc

    • PixCell II

    • AutoPix


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Arcturus PixCell II


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Arcturus AutoPix


Uv lm

UV LM


Uv lm1

UV LM

  • Laser Microdissection (LMD/Leica)

  • Cut and catapulting system (PALM/Zeiss)

  • Mmi-CellCut (MMI AG)


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LMD/Leica


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PALM/Zeiss


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Mmi-CellCut (MMI AG)


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LM

  • UV IR :

    • Arcturus Veritas

    • Acturus XT


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Arcturus Veritas

Acturus XT


Eppendorf ppmd microdissector

: Eppendorf PPMD microdissector

- , -


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-


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  • LM , ,


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  • LM , ,

  • FFPE,

  • : , ,


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  • 1 = 6-7 pg

  • 1 = 10-30 pg

    • 80-85% rRNA

    • 15-20% tRNA, snRNA

    • 1-5% mRNA


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  • ( UV LM), ,

  • ,

  • UV IR ,


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  • ( , ).


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  • contact-lift off ,

  • FFPE , => PCR -

  • IR LCM - 10m


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LM

  • :

    • -

    • ,


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Touch Prep

Thin Prep

Cyto Spin

FISH

LM


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LM


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  • LOH


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- LOH

  • LOH

  • LOH :


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- X


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  • Western Blot

  • 2D-PAGE


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LM


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inner cell mass cells ( )

( )


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LCM


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LCM Arabidopsis

embryo


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LCM


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LCM

LCM

LCM

LCM

LCM

LCM


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LM

  • STR -

    • => ->


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  • :

  • - LM


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  • -

    • ( ),

  • - STR


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    • 70% ,

    • 1-2%

    • . ,


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  • Horsman et al-

    • 25% ( )


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,

15-20 , LM.


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  • -

    • LCM- FISH-

  • ( )


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LM

  • - ( )

  • -

  • LCM , , , ,


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LM

  • , , .


Lm expression microdissection xmd automated cell recognition

LM- Expression Microdissection (xMD), Automated Cell Recognition

  • - , -


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    • . ,

  • 50 000

  • -


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!


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!


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