-
Sponsored Links
This presentation is the property of its rightful owner.
1 / 81

Център по молекулна медицина PowerPoint PPT Presentation


  • 131 Views
  • Uploaded on
  • Presentation posted in: General

Лазерната микродисекция- безценният инструмент на молекулярната патология и диагностика, съдебната медицина и съвременната наука. Център по молекулна медицина. Нова биомедицинска революция.

Download Presentation

Център по молекулна медицина

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


- ,



,


:

  • : , , ( ),


.....................



?

    • : , ,

    • : , , . , , ( ) . ,


?

  • -

  • - , , - - . .


  • LM ( )

    • Lowry and Passonneau-

    • Goelz et al-


,


(LM)

  • Meier-Ruge et al- 1976, LM UV , , -

  • Shibata- 1993-UV , (Selective ablation of unwanted regions)

  • Emmert-Buck et al- 1996 NIH- Arcturus Engineering


LM

  • /


LCM


LM

  • IR LCM- EVA , .

  • UV LM- ,

  • IR UV- Arcturus Veritas Acturus XT


IR-LCM


LCM

LCM

LCM


IR LCM

  • Arcturus/MDS, Inc

    • PixCell II

    • AutoPix


Arcturus PixCell II


Arcturus AutoPix


UV LM


UV LM

  • Laser Microdissection (LMD/Leica)

  • Cut and catapulting system (PALM/Zeiss)

  • Mmi-CellCut (MMI AG)


LMD/Leica


PALM/Zeiss


Mmi-CellCut (MMI AG)


LM

  • UV IR :

    • Arcturus Veritas

    • Acturus XT


Arcturus Veritas

Acturus XT


: Eppendorf PPMD microdissector

- , -


-


  • LM , ,


  • LM , ,

  • FFPE,

  • : , ,


  • 1 = 6-7 pg

  • 1 = 10-30 pg

    • 80-85% rRNA

    • 15-20% tRNA, snRNA

    • 1-5% mRNA


  • ( UV LM), ,

  • ,

  • UV IR ,


  • ( , ).


  • contact-lift off ,

  • FFPE , => PCR -

  • IR LCM - 10m


LM

  • :

    • -

    • ,


Touch Prep

Thin Prep

Cyto Spin

FISH

LM


LM


  • LOH


- LOH

  • LOH

  • LOH :


- X



  • Western Blot

  • 2D-PAGE


LM



inner cell mass cells ( )

( )


LCM


LCM Arabidopsis

embryo


LCM


LCM

LCM

LCM

LCM

LCM

LCM


LM

  • STR -

    • => ->


  • :

  • - LM


  • -

    • ( ),

  • - STR


    • 70% ,

    • 1-2%

    • . ,


  • Horsman et al-

    • 25% ( )


,

15-20 , LM.


  • -

    • LCM- FISH-

  • ( )


LM

  • - ( )

  • -

  • LCM , , , ,


LM

  • , , .


LM- Expression Microdissection (xMD), Automated Cell Recognition

  • - , -


    • . ,

  • 50 000

  • -


!


!


  • Login