1 / 13

Viral Metagenome Project

Viral Metagenome Project. Hyeon J. Lee BNFO 301. My Claimed sequences. Bphs.aoix928-b2 997 Bphs.aoix928-b4 956 Bphs.aoix928-g2 960 Contiguous-sequence Phage Bearpaw hot springs. SEQUENCE-SIMILAR-TO ALIGNMENT-OF. Overlaps. Using only this part to find the overlaps.

gzeno
Download Presentation

Viral Metagenome Project

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Viral Metagenome Project Hyeon J. Lee BNFO 301

  2. My Claimed sequences • Bphs.aoix928-b2 997 • Bphs.aoix928-b4 956 • Bphs.aoix928-g2 960 • Contiguous-sequence • Phage Bearpaw hot springs

  3. SEQUENCE-SIMILAR-TO ALIGNMENT-OF

  4. Overlaps Using only this part to find the overlaps

  5. My Claimed Sequence 997 1 BPHS.AOIX928-b2 962 BPHSe.AOIX4443-b2 1 969 BPHSe.AOIX737-b4 1 855 898 BPHSe.AOIX2604-b2 1 716 910 BPHSe.AOIX709-g2 996 813 1 BPHSe.AOIX1005-g2 954 879 1 BPHSe.AOIX3245-g2 917 884 1 BPHSe.AOIX480-g2 926 898 1 BPHSe.AOIX3125-g2 981 925 1 1 1889

  6. My new sequence GGGAATTTAAAGGATAGCTTCTGAGATATCAGCTTGAATTCGTTGATGCGGCCGCTTTAGATACAAGCGGCCGCATCGACGAATTCTCTAGATATCGCTCAATACTGACCATTTAAATCATACCTGACCTCCATAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGATCGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCTATGAGCCATATTCAACGGGAAACGTCTTGCTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGGCTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCAGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAACATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACGGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGGTGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGCTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTGACGAGGGGAAATAATAGGTTGTATTGATGTTGAACGAGTCCGAATCCCAGACCCAAACCCGGAATCTTGCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCACTTGATGCTCGATGAGTTTTTCTGAGGGCCCAAATGTAATCACCTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGATGCTCCCGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATTTTCTACCGAAGAAAGGCCCACCCGTGAAGGTGAGCCAGTGAGTTGATTGCAGTCCAGTTACGCTAAATTAATACGACTCACTATAGGGGAAGATATCGTCGACGAAGTCATCAAGGATCCAATCGCGCCCAGTTAGATCCCCCCAAAA

  7. Cloning vector pSMARTGC HK Cloning vector pSMART-HCKan

  8. 1 1889 350 1050 1400 1750 700 Broad host range vector pBTB-2 E-value=0, %ID=97.38% Broad host range vector pBTL-2 Broad host range vector pBMTL-2 Broad host range vector pBMTB-2 Broad host range vector pBT-2 Aminoglycoside antibiotic It causes a frameshift mutation or it prevents the translation of RNA Kanamycin resistance gene from TN903

  9. 1 1889 350 1050 1400 1750 700 Function : Kanamycin resistance Proteins: Aminoglycoside phosphotransferase E-value=5.88 x 10^133, %ID=98.56% Aminoglycoside 3’-phosphotransferase E-value=1.87 x 10^132, %ID=97.61% Tn903 aminoglycoside phosphotransferase E-value=4.16 x 10^132, %ID=97.13% Kanamycin phosphotransferase E-value=5.43 x 10^132, %ID=97.13% Organisms: Cloning vector pSMART-LCKan from 1 to 209 Corynebacterium diphtheriae from 1 to 209 Synthetic construct from 1 to 209

More Related