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Study of Malathion's Toxicity to Mollusk and Its Implications with Cancer Development

Study of Malathion's Toxicity to Mollusk and Its Implications with Cancer Development. Caroline Hsiao Manhasset Science Research In C ooperation With: Dr . Wei Zhu SUNY College at Old Westbury SUNY Old Westbury Neuroscience Research Institute. Cancer Rate per 100,000 . Need.

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Study of Malathion's Toxicity to Mollusk and Its Implications with Cancer Development

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  1. Study of Malathion's Toxicity to Mollusk and Its Implications with Cancer Development Caroline Hsiao Manhasset Science Research In Cooperation With: Dr. Wei Zhu SUNY College at Old Westbury SUNY Old Westbury Neuroscience Research Institute

  2. Cancer Rate per 100,000 Need Figure 1: Graph of Cancer Incidence Worldwide http://info.cancerresearchuk.org/cancerstats/types/liver/incidence/

  3. Low Level (Endogenous) Morphine High Level of Morphine Morphine Signaling Nitric Oxide Healthy state, Antiviral, Antibacterial Cellular Damage, Cancer Development Figure 2: Basic outline of morphine signaling. Diagram Created by Author

  4. Malathion usage 2010 Malathion Figure 3: Increased usage of Malathionin recent years Malathion Detected After Spraying Malathion usage 2007 http://10.7.1.5/cgi-bin/overmain?args=d29ya3N0YXRpb249MTAuMjAuMS43NSZ1cmw9YUhSMGNEb3ZMM2QzZHk1d1pYTjBjSEp2WkhWamRITnZibXhwYm1VdVkyOXRMMHhoWW1Wc2N5MU5VMFJUTDBFeE16Uk5MbkJrWmc9PSZwcm9maWxlPVN0dWRlbnREZWZhdWx0JnVzZXI9TUFOSEFTU0VUXGNhcm9saW5lX2hzaWFvJm9sPTEmcmE9MSZhbD0wJnJhdGluZ3M9c3NocA==

  5. Mytilusedulis Posterior duct muscle – where hemocytes (immune cells) were dissected Pedal ganglia was dissected Figure 5: examples of Mytilusedulis dissection

  6. Purpose and Hypothesis Figure 6: The purpose of this study was to observe Malathion ‘s effect on Mytilusedulis Hypothesis: H(a): Malathion will effect the opiate signaling pathway in hemocyte cells and therefore may show a link to cancer development. H(0): Malathion will have no effect on the opiate signaling pathway in hemocytecells and show no correlation to tumor development.

  7. H(a): Malathion will effect the opiate signaling pathway in hemocyte cells and therefore may show a link to cancer development. H(0): Malathion will have no effect on the opiate signaling pathway in hemocyte cells. Hypothesis

  8. Mytilusedulis Dissection N = ~1,000 per group Method – Image Analysis Hemocyte Extraction Divide Hemocytesinto Two Six-Well Plates Morphine and Naloxone 10-6M Malathion and Naloxone 10-6M Malathion and L-name 10-6M Morphine and L-name 10-6M Control 10-6M Morphine 10-6M Malathion10-6M Add 15uL of Trypan Blue Stain Take Pictures Take Pictures Calculate and Analyze Form Factor Count Dead Cells Statistical Analysis (ANOVA With SceffePosthoc Test)

  9. Search for fourteen genes of interest relating to the nAChR (CHRND, CHRNA4, CHRNA1, CHRNA10, CHRNA9, CHRNB3, CHRNA2, CHRNB2 CHRNB1, CHRNE, CHRNA1, CHRNA3, CHRNB3, CHRNA6) Method – Microarray Analysis Collect signal strength from past microarray study Calculate percent change in gene expression as compared to control. Statistical Analysis (ANOVA With SceffePosthoc Test)

  10. N = ~1,000 per group Cells are pretreated with 125I labeled morphine Method - Morphine Release Assay Malathion (10-4M, 10-5M, 10-6M, 10-7M, 10-8M) Control (Nicotine 10-6M) Enter Samples to Gammar Counter Analyze Concentration of Morphine Released

  11. Image Analysis Image Analysis Picture Taken by Author Normal Hemocytes Hemocytes with Malathion Exposure

  12. Results Malthion10-6M Naloxone 10-6M Morphine 10-6M Naloxone 10-6M Malthion10-7M Malthion10-6M Malthion10-5M Morphine 10-6M Malthion10-3M N = ~1,000 per group Graph Created by Author

  13. Results * ^ * ^ N = ~1,000 per group * ^ * ^ ^ ^ ^ ^ N = ~1,000 per group Graph Created by Author

  14. N = ~1,000 per group Results 10-8M 10-7M Control 10-6M 10-4M 10-5M Graph Created by Author

  15. Results Graph Created by Author

  16. Inhibition of Acetylcholinesterase Kills Insects Malathion Disruption of Opiate Signaling in Animals and Humans Discussion Inappropriate Nitric Oxide Release Oxidative Stress Potential Tumor Development Diagram Created by Author

  17. Conclusion • Malathion is toxic to Mytilusedulis • Affects the opiate signaling system in both animals and humans.

  18. Future Studies Diagram Created by Author

  19. Acknowledgements • Dr. Wei Zhu - SUNY Old Westbury Neuroscience Research Institute • Mr. Peter Guastella - Manhasset High School • The SCA - Summer Studies Scholarship

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