Fast Electrical Impedance Spectroscopy measurement techniques for dynamic
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Fast Electrical Impedance Spectroscopy measurement techniques for dynamic bioimpedance characterization. Ph.D. Student: B.Sánchez Thesis Advisor: R.Bragós. Electronic Engineering Department Universitat Politècnica de Catalunya. Contents. 1.- Introduction 2.- Motivation

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Electronic engineering department universitat polit cnica de catalunya

Fast Electrical Impedance Spectroscopy measurement techniques for dynamicbioimpedance characterization.Ph.D. Student: B.SánchezThesis Advisor: R.Bragós

Electronic Engineering Department

Universitat Politècnica de Catalunya


Electronic engineering department universitat polit cnica de catalunya

Contents

1.- Introduction

2.- Motivation

3.- Multitone Burst Signal

4.- Results

5.- Conclusions


Electronic engineering department universitat polit cnica de catalunya

Introduction

Linear Time Invariant system: Impedance

Transfer Function

Laplace/Fourier Transform

1


Electronic engineering department universitat polit cnica de catalunya

Ro

|Z|

R

8

f

fc

Introduction

Biologicalsystems: Bioimpedance

EquivalentCircuit

Cellmembrane

Extracellularmatrix

LF

HF

2


Electronic engineering department universitat polit cnica de catalunya

Introduction

Ex-vivo tissuecharacterization:

3


Electronic engineering department universitat polit cnica de catalunya

Introduction

In-vitro staticcellculturegrowthcharacterization:

4


Electronic engineering department universitat polit cnica de catalunya

Motivation

Challenge (I): In-vitro myocites cell culture growing characterization for tissue engineering applications

Link video

5


Electronic engineering department universitat polit cnica de catalunya

Motivation

Challenge (II): In-vivo myocitespatchmonitoring and characterization:

6


Electronic engineering department universitat polit cnica de catalunya

Multitone Burst Signals

Time domain:

7


Electronic engineering department universitat polit cnica de catalunya

Multitone Burst Signals

Frequency domain:

8


Electronic engineering department universitat polit cnica de catalunya

Multitone Burst Signals

Crest Factor:

Schroeder:

Newman:

Genetic Algorithm:

9


Electronic engineering department universitat polit cnica de catalunya

Multitone Burst Signals

Frequency Plan:

Avoid intermodulation products: shift original tones for BW guard.

10


Electronic engineering department universitat polit cnica de catalunya

Multitone Burst Signals

Multitone bursts with Bilateral Quasi Logarithmic Frequency Distribution:

11


Electronic engineering department universitat polit cnica de catalunya

Results

Measurement Hardware Setup:

  • Custom FPGA based arbitrary signal generator.

  • DAC 12bits, 16Msamples, 40Ms/s, 1st order hold.

  • Ethernet capable controller.

  • PXI solution

  • PXI-5422: AWG, 200Ms/s, 32MB,16 bits.

  • PXIe-5122: AD 2channels, 100Ms/s, 64MB/channel, 14bits.

  • PXI-2530: multiplexation matrix, 128 channels.

12


Electronic engineering department universitat polit cnica de catalunya

Results

Yeast cell suspension settlement characterization:

Estimation of the Growth Cell Curve

13


Electronic engineering department universitat polit cnica de catalunya

Conclusions

  • Non invasive and non destructive technique for passive electrical properties charaterization of biological materials.

  • Valid measurement method for cell growth estimator and suitable for dynamic biological materials and systems characterization.

  • Wide application areas: electroporation, tomography, cell counting in microfludic, biosensors, tissue engineering,..

  • The final application field is the cardiac tissue characterization in regenerative medicine applications.

14


Electronic engineering department universitat polit cnica de catalunya

Fast Electrical Impedance Spectroscopy measurement techniques for dynamicbioimpedance characterization.Ph.D. Student: B.SánchezThesis Advisor: R.Bragós

Electronic Engineering Department

Universitat Politècnica de Catalunya


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