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To Rerack the Spectrum Library, Three programs will be run. SpecReRack1

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To Rerack the Spectrum Library, Three programs will be run. SpecReRack1 Moves 50 ul to Storage plate ABGene0781_384Storage Spec Aliquot Dispenses 10 ul from storage plate to three screening plates ABGene1055_384 Dispenses 3 ul into Plate with 72 ul of DMSO for diluted plate

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slide1

To Rerack the Spectrum Library, Three programs will be run.

  • SpecReRack1
  • Moves 50 ul to Storage plate
  • ABGene0781_384Storage
  • Spec Aliquot
      • Dispenses 10 ul from storage plate to three screening plates
  • ABGene1055_384
  • Dispenses 3 ul into Plate with 72 ul of DMSO for diluted plate
  • SpecRerack 70 ul
  • Moves the remaining Chemical Library to Storage plate.
slide2

Turn On instrument and hotel.

Check Deck of instrument and remove all tip boxes and plates ect.

Open Biomek Software

Be sure gripper UP

Home all axes.

slide3

Thaw libraries (about 1 hour)

Centrifuge briefly.

Open SpecReRack1

Set up Deck (red arrow)

Hit start (Blue Arrow)

slide4

Check deck again

All errors in deck set up will crash the robot. So check Again!

Run program

slide5

Close program

Remove used tips

Spin Storage plate to remove bubbles

Open Spec Aliquot

Set up deck

slide6

Run Spec Aliquots program

Check deck again when prompted

slide7

Run SpecRerack 70 ul

Quick spin for all plates to put all chemicals at bottom of well.

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