Excitatory effect of oxytocin on uterine smooth muscle
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Experiment for pharmacology for reproductive system. Excitatory effect of oxytocin on uterine smooth muscle. Shi-Hong Zhang (张世红) Dept of Pharmacology shzhang713@zju.edu.cn. Uterine stimulants. Pituitrin ( 垂体后叶素 ) Oxytocin ( 催产素 ) Ergot alkaloids ( 麦角生物碱 ) Prostaglandins (PGs, 前列腺素 ).

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Excitatory effect of oxytocin on uterine smooth muscle

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Excitatory effect of oxytocin on uterine smooth muscle

Experiment for pharmacology for reproductive system

Excitatory effect of oxytocin on uterine smooth muscle

Shi-Hong Zhang (张世红)Dept of Pharmacologyshzhang713@zju.edu.cn


Excitatory effect of oxytocin on uterine smooth muscle

Uterine stimulants

  • Pituitrin (垂体后叶素)

  • Oxytocin (催产素)

  • Ergot alkaloids (麦角生物碱)

  • Prostaglandins (PGs, 前列腺素)


Excitatory effect of oxytocin on uterine smooth muscle

A. Uterine stimulants

Pituitrin(垂体后叶素)

  • oxytocin

  • vasopressin


Oxytocin oxt
Oxytocin (OXT, 催产素)

Uterine stimulants

1. Pharmacological effects:

(1) Uterus:

Elevating both the frequency and force of uterine contraction, at rhythm similar to natural labor, highly dependent on estrogen level and condition of uterus.

(2) Breast: milk ejection.

(3) Blood vessel: vasodilation at large dose.


Oxytocin oxt1
Oxytocin (OXT, 催产素)

Uterine stimulants

2. Clinical uses:

-- to expedite delivery and induced labor;

-- to reduce the incidence and extent of postpartum hemorrhage.


Oxytocin oxt2
Oxytocin (OXT, 催产素)

Uterine stimulants

3. Adverse effects:

-- Intrauterine asphyxia or uterine rupture (overdose)

-- Allergy

4. Contraindications:

--hypertonic dysfunction of uterus

-- abnormality in birth canal

-- abnormal fetal position


Excitatory effect of oxytocin on uterine smooth muscle

Uterine stimulants

Ergot alkaloids(麦角生物碱)Ergometrine(麦角新碱),Ergotamine(麦角胺), Ergotoxine(麦角毒)

1. Pharmacological effects:

(1) Uterus (ergometrine):

-- strongly stimulating uterine smooth muscle, both the body and the cervix; ready to induce tonic contraction at larger dose .

(2) Blood vessel (ergotamine):

-- contracting peripheral blood vessel and cerebral vessel.

(3) blocking  receptor (ergotoxine):


Excitatory effect of oxytocin on uterine smooth muscle

A. Uterine stimulants

Ergot alkaloids(麦角生物碱)

2. Clinical uses:

(1) Endometrorrhagia (子宫出血), and puerperal replacement of uterus (产后子宫复位) : ergometrine;

(2) Migraine (偏头痛) : ergotamine;

(3) Hibernation therapy (冬眠疗法) : dihydroergotoxine + pethidine + promethazine.


Excitatory effect of oxytocin on uterine smooth muscle

A. Uterine stimulants

Ergot alkaloids(麦角生物碱)

3. Adverse effects:

-- nausea, vomiting, BP ;

-- allergic reaction;

-- prohibited in expediting delivery and induced labor.


Excitatory effect of oxytocin on uterine smooth muscle

Uterine stimulants

Prostaglandins (PGs, 前列腺素)

Dinoprostone (地诺前列酮, PGE2)

Dinoprost (地诺前列素,PGF2)

1. Pharmacological effect:

stimulating uterine smooth muscle.

2. Clinical uses:toinduce labor

2. Adverse effects: GI reaction, attack of asthma (PGF2); glaucoma (PGE2).


Objectives
Objectives

  • To observe the excitatory effect of oxytocin on uterine smooth muscles and its dose dependent manner.


Methods
Methods

  • Estrodiol pretreatment (0.2mg) on rats (200 g  40g) 24hr before experiment;

  • Prepare uterus smooth muscle specimen.


Methods1
Methods

Uterus of the rat


Methods2
Methods

Uterus of the rat


Methods3
Methods

Perfusion system


Methods4
Methods

  • Set parameters of recording system:

  • Input pattern: tensility

  • Gain: 200-500

  • Lower frequency: direct current

  • Upper frequency: 10 Hz

  • Sampling interval: 2 ms

  • Preload: 1g


Methods5
Methods

  • The specimen is stabilized in the perfusion chamber for 15-30 min until the contractive force and rhythm become regular. Record the basal contractive force and frequency (optional).

  • Add 0.02ml, 0.04ml, 0.14ml oxytocin (0.01U/ml) into the perfusion system. Record the maximal force and frequency (optional) of contraction at each drug concentration accordingly.


Methods6
Methods

  • Add 0.04ml, 0.14ml oxytocin (0.1U/ml) into the perfusion system. Record the maximal force and frequency (optional) of contraction at each drug concentration accordingly.

  • Add 0.04ml, 0.14ml oxytocin (1U/ml) into the perfusion system. Record the maximal force and frequency (optional) of contraction at each drug concentration accordingly.


Methods7
Methods

  • Add 0.04ml, 0.14ml oxytocin (10U/ml) into the perfusion system. Record the maximal force and frequency (optional) of contraction at each drug concentration accordingly.


Methods8
Methods

Notice:

1.Avoid injury and excessive stretch of uterus during the whole process of preparation.

2.Control the gas bubbles in a proper rate in case of impact on uterus contraction.

3.Record a period of baseline of contraction before drug treatment in order to compare that before and after.

4.Add drug in the order as prescribed.


Lab report
Lab report

1.题目

2.署名: 作者和合作者姓名及单位。

3.结构式摘要(目的,方法,结果,结论)。

4.材料和方法: 主要实验材料,标本制备方法,仪器装置连接和仪器参数,实验数据记录条件,观察指标。

5.结果: 实验结果的文字性描述,作量效曲线。

6.讨论: 回答思考题。


L earning issue
Learning issue

1.缩宫素对子宫的作用及特点。

2.为什么实验前用雌二醇处理动物?

3. 缩宫素与麦角新碱对子宫平滑肌的作用特点有何不同?


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