1 / 4

0.1

Supplementary Figure S1. A. A375. A375R2. Vemurafenib (µM). 0. 0.1. 1. 10. 0. 0.1. 1. 10. BRAF (Full Length). p61-BRAF Isoform. pMEK. pERK. B. Tubulin. A375. A375-R4 NRas Q61K. Vemurafenib (µM). Tubulin. Ras. pS338-cRaf. pMEK. pERK. Supplementary Figure S2.

gotzon
Download Presentation

0.1

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Supplementary Figure S1 A A375 A375R2 Vemurafenib (µM) 0 0.1 1 10 0 0.1 1 10 BRAF (Full Length) p61-BRAF Isoform pMEK pERK B Tubulin A375 A375-R4 NRas Q61K Vemurafenib (µM) Tubulin Ras pS338-cRaf pMEK pERK

  2. Supplementary Figure S2 Vemurafenib Vehicle Vemurafenib LY2835219

  3. Supplementary Figure S3 A375 A375-RV2 Vemurafenib (µM) pERK Tubulin

  4. Supplementary Figure S4 A %G1= 54.25 %S = 34.96 %G2/M = 10.79 %G1= 82.10 %S = 2.73 %G2/M = 15.17 %G1= 74.95 %S = 3.81 %G2/M = 21.24 M14 300 %G1= 58.58 %S = 24.88 %G2/M = 16.54 250 A375-R1 200 M14-R M14-R Relative Caspase 3 Activity (%) 150 100 M14 A375 DMSO 1.5mM 5mM 50 LY2835219 B 0 %G1= 38.71 %S = 35.28 %G2/M = 26.02 0.0 2.6 %G1= 55.23 %S = 13.34 %G2/M = 31.43 3.0 3.5 4.0 Log Concentration LY2835219 (nM) %Debris = 72.17 %Debris = 41.73 A375-RV1 A375-RV2 A375-RV1 A375-RV2 A375-R1 A375-R1 C A375 A375 Cyclin D1 ←Cyclin D2 Cleaved-PARP Tubulin Cyclin D1 siRNA

More Related