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生物化学实验 CQMU PowerPoint PPT Presentation


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生物化学实验 CQMU. 双缩脲法测定血清蛋白质含量. P 、 51 生物化学与分子生物学实验室 2008.09. 蛋白质的定量分析是生物化学和其他生命学科最常涉及的分析内容,是临床上诊断疾病及检查康复情况的重要指标,也是许多生物制品,药物、食品质量检测的重要指标。在生化实验中,对样品中的蛋白质进行准确可靠的定量分析,则是经常进行的一项非常重要的工作。. 目前测定蛋白质含量的方法有很多种,下面列出根据蛋白质不同性质建立的一些蛋白质测定方法: 物理性质:紫外分光光度法。 化学性质:凯氏定氮法、双缩脲法、 Lowry 法 染色性质:考马斯亮蓝染色法、银染法。

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生物化学实验 CQMU

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Cqmu

CQMU

P51

2008.09


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Lowry


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  • Biuret reaction

H2N-OC-NH-CO-NH2Cu2+


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-CO-NH-Cu2+Cu2+-


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  • 120 mg

  • -CONH--CONH2-CH2NH2 -CS-NH2


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I0

It

C

b

  • IoIt


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It

T

=

*100%

Io

Io

A = -T

=

It

  • TransmittanceTItI0

  • AbsorbanceA:

A


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  • Lambert-Beer

AKCL

A

K

C

L

AKL


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  • DNA A260/A280=1.8 )


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  • Lambert-Beer

  • 100%A-C


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  • A-C

  • A =Kcb

  • A =K c b

  • c =A /A c


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  • 20

  • 0%T

  • 100%T100%

  • MODE(A)

  • 100%TA


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  • 13

  • 30540 nm


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  • g/L


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  • 2030 min4


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  • Login