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抗 体. 第四节 淋巴细胞抗原识别受体的 编码基因及多样性的产生. n ( 抗原表位 ) ↑↓ n(T/B 细胞克隆 ) ← n(TCR/BCR) ← n(V 基因 ) 机体存在着特异性识别各种抗原的 T 、 B 细胞克隆,称 T 、 B 细胞库 , 即 TCR 、 BCR 的多样性. 一个机体每一个淋巴细胞是不是具有这么巨大数量的 V 基因库呢? 没有。 研究证明:

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n()

n(T/B) n(TCR/BCR) n(V)

TBTB, TCRBCR


V

V()VBCR1014TCR1016


BCRTCR

  • V

    V C

  • VVDJ

  • VVJ

  • CDR1+CDR2VCDR3DCDR3J


14

CV1~9HC9

BCR/IgV VDJV VJCDR1+CDR2VCDR3DCDR3J

2

22

BACK


  • CV1~9HC9

  • TCRVDJ VJ

  • VDJ

    V


14

7q

TCRVDJ VJ

7p

BACK


1DNADNA

2

(1)

BT


(2)

L222/=6535


3()

BIg

VC


CH9CDNAC


4BCR(Ig)Ig

SC()

MC()


SC()MC()


1


2

CDR3VJVDJ(N)DNAV-D-JVNDNJ CDR3


3

BV(CDR3)IgVCDR()


Burnet() 1957

( ,Clone),


V

(IgG, IgE) 2

(IgA) 4

(IgM) 105

Ag

1.

2.


C

1.

Ab(IgMIgG) + Ag C1q

IgG4IgA

2.

(1)(opsonization)IgG + () FcR

(2)ADCCIgG + () Fc RNK

(3)

3.


IgG

1.

(1)

(2)

(3)(75%Ig)

(4)(2123)

(5)35(8.017 mg/ml)

(6)SPA


2.

(1)

(2)

(3)

(4)ADCC

3.

(1)

(2)

(3)()

(4)

(5) IgG

(6)


IgM

1.(900 kd)

2.Ig

3. Ig

4.Ig510%

5. 5 IgM

6.

7.IgM IgM

8.BIgM(mIgM)

9.


IgA1

IgA2,

IgA

1.

2. 6

3.Ig515%

4.

5.IgA


Y

Y

Y

Y

Y

Y

Origin of Secretory Component of sIgA


IgD

1.

2.

B,

3.

1B

2

4.Ig1%

5. 3


IgE

1.

2.(Ig0.002%)

3. 3

4.

5.

6.

IgE


(polyclonal antibody)

1. B:

2.

1

2


(monoclonal antibody, McAb)

1.B

2.

3.

B

4.

HAT(H), (A)

(T)


  • McAb


  • hybrid cell


DNA

1.H

HGPRT

2.

A-

TK

3.T


HAT

  • HAT

    hypoxanthine,H)

    aminopterin,A):

    thymidine,T)


B B 1. Ig+) 1. 2.HGPRT+HAT 2.8-AGHGPRT-PEG HAT - HGPRT+Ig+


-

-


B

HAT

3-5

McAb

McAb

McAb


1

2

3

4

5

6 McAB

7


  • B

  • 10-15ug/100ul+ 2-4 3

  • 6-12Balb/c


  • ()


  • IgHGPRT

  • NS1SP20X63

  • 8-

  • 95

  • =31


  • B-3

  • =15110

  • 40%PEG1000-2000

  • 24HAT 2 HT 2


  • 4-5


  • 1/10


  • 4-5


  • 17-10



  • 17-10



  • 1

  • 2

  • 3

  • 4

  • 5


  • 1.

  • 2.

  • 3.,


  • :

  • B


  • IcTnIcTnI


(genetic engineering antibody)

1. DNAIg

2. -(chimeric antibody)

(reshaped humanized antibody)

(bispecific antibody)


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