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gfp -gene as cDNA in a host-DNA-fragment E. coli (new host)

?. gfp -gene as cDNA in a host-DNA-fragment E. coli (new host). ???????.  . gfp. Aequorea victoria (donor) . host. ?. host. gfp -gene as cDNA in a host-DNA-fragment DNA - transport system E.coli (new host).  . gfp. host. 1. 3. 4. gfp.

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gfp -gene as cDNA in a host-DNA-fragment E. coli (new host)

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  2. gfp-gene as cDNA in a host-DNA-fragmentE. coli (new host) ???????  gfp Aequorea victoria (donor) host ?

  3. host gfp-gene as cDNA in a host-DNA-fragmentDNA-transport system E.coli (new host)  gfp

  4. host 1 3 4 gfp gfp-gene as cDNA in a host-DNA-fragmentDNA-transport system E.coli (new host)  gfp purified cloning vector DNA preparation of DNA-incor- porating cells cleavage of the interesting gene with appr. restriction enzymes

  5. host 1 3 4 gfp??? gfp-gene as cDNA in a host-DNA-fragmentDNA-transport system E. coli (new host)  gfp purified cloning vector DNA preparation of DNA-incor- porating cells cleavage of the interesting gene with appr. restriction enzymes identification/verification??? ? ?

  6. host 2 3 4 4 1 3 gfp gfp-gene as cDNA in a host-DNA-fragmentDNA-transport system E. coli (new host)  gfp purified cloning vector DNA preparation of DNA-incor- porating cells (competent cells) cleavage of the interesting gene with appr. restriction enzymes recovery of gfp-fragment from agarose gel electrophoresis gfp cleavage of vector DNA importation of constructed DNA (transformation) gfp ? gfp joining of vector DNA and gene fragment (ligation) cultivation of cells

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