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CHMI 2227E Biochemistry I

CHMI 2227E Biochemistry I. Enzymes: catalysis. Enzyme catalysis. General acid/base catalysis :. Covalent catalysis :. Enzyme catalysis Basic reaction mechanisms. Three main types of catalysis are found:. Metal ion catalysis :. Chymotrypsin. O. OH. +. R. HOR. H.

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CHMI 2227E Biochemistry I

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  1. CHMI 2227EBiochemistry I Enzymes: catalysis CHMI 2227 - E.R. Gauthier, Ph.D.

  2. Enzyme catalysis CHMI 2227 - E.R. Gauthier, Ph.D.

  3. General acid/base catalysis: Covalent catalysis: Enzyme catalysisBasic reaction mechanisms • Three main types of catalysis are found: • Metal ion catalysis: CHMI 2227 - E.R. Gauthier, Ph.D.

  4. Chymotrypsin O OH + R HOR H Enzyme catalysisGeneral acid/base catalysis CHMI 2227 - E.R. Gauthier, Ph.D.

  5. H+ Enzyme catalysisMetal ion catalysis CHMI 2227 - E.R. Gauthier, Ph.D.

  6. Nucleophile Enzyme catalysisCovalent catalysis CHMI 2227 - E.R. Gauthier, Ph.D.

  7. Enzyme catalysisRefresher – nucleophiles and electrophiles CHMI 2227 - E.R. Gauthier, Ph.D.

  8. General Acid/ base catalysis Covalent catalysis Enzyme catalysis- Enzymes often combine two catalytic strategies CHMI 2227 - E.R. Gauthier, Ph.D.

  9. Example of enzyme catalysis1. Chymotrypsin CHMI 2227 - E.R. Gauthier, Ph.D.

  10. Example of enzyme catalysis1. Chymotrypsin CHMI 2227 - E.R. Gauthier, Ph.D.

  11. Example of enzyme catalysisImportance of the catalytic triad CHMI 2227 - E.R. Gauthier, Ph.D.

  12. Example of enzyme catalysis1. Chymotrypsin - specificity CHMI 2227 - E.R. Gauthier, Ph.D.

  13. Ser195 Ser195 Example of enzyme catalysis1. Chymotrypsin - specificity CHMI 2227 - E.R. Gauthier, Ph.D.

  14. Catalytic triad Substrate Peptide bond cleavage C-ter part of the protein Enzyme regeneration N-ter part of the protein Example of enzyme catalysis1. Chymotrypsin - catalysis Covalent intermediate CHMI 2227 - E.R. Gauthier, Ph.D.

  15. Example of enzyme catalysis1. Chymotrypsin - catalysis CHMI 2227 - E.R. Gauthier, Ph.D.

  16. Example of enzyme catalysisSimilarities between different proteases CHMI 2227 - E.R. Gauthier, Ph.D.

  17. Example of enzyme catalysisSimilarities between different proteases CHMI 2227 - E.R. Gauthier, Ph.D.

  18. Example of enzyme catalysis2. Enolase CHMI 2227 - E.R. Gauthier, Ph.D.

  19. Example of enzyme catalysis2. Enolase CHMI 2227 - E.R. Gauthier, Ph.D.

  20. Example of enzyme catalysis2. Enolase CHMI 2227 - E.R. Gauthier, Ph.D.

  21. Example of enzyme catalysis3. Lysozyme CHMI 2227 - E.R. Gauthier, Ph.D.

  22. Sugar portion of peptidoglycan Example of enzyme catalysis3. Lysozyme CHMI 2227 - E.R. Gauthier, Ph.D.

  23. Example of enzyme catalysis3. Lysozyme CHMI 2227 - E.R. Gauthier, Ph.D.

  24. H2O 2 Example of enzyme catalysis3. Lysozyme CHMI 2227 - E.R. Gauthier, Ph.D.

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