The development of a RNA-sequencing pipeline based on tuxedo tools. Martijn Derks Masoed Ramuz Nick Alberts Rico Hagelaar. Index . Dataset Pipeline 1 ( Tophat_cuff ) Pipeline 2 ( Cuff_diff ) Pipeline 3 (Summary) Conclusions Future prospects. Dataset.
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The development of a RNA-sequencing pipeline
based on tuxedo tools
Ganet al. 2011. Multiple reference genomes and transcriptomes for Arabidopsis thaliana. Nature. 477, P 419–423.
Input data (FastQ)
Total intron length
Basic for plot R
Control vs condition
Functions + enrichment
GC genes vs FPKM
CV= STDEV/ Average
Abscisic acid biosynthesis (stress conditions)
1. Cold, salinity and drought stresses: An overview
2. Cold stress regulation of gene expression in plants
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