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Supplementary Fig. S2 Sequence comparison of DFR genes. DNAs of DFR genes from N. tomentosiformis, N. sylvestris,N. tabacum cv. BY-4, and N. tabacum cv. Xanthi were cloned and sequenced, and then sequences were aligned using CLUSTAL W. One-base spontaneous deletion in NtDFR1 cv. Xanthi is highlighted in red.