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The Phot oblea ch i ng Eff ect by Stephen Payne ?. Binds to double-stranded DNA and fluoresces. Does not bind to single-stranded DNA. SYBR Green. The Problem. LED. Double-Stranded DNA. Bleached SYBR Green. Light. Fluorescent SYBR Green. Suspicion of The Problem.
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ThePhotobleachingEffect by Stephen Payne ?
Binds to double-stranded DNA and fluoresces Does not bind to single-stranded DNA SYBR Green
The Problem LED Double-Stranded DNA Bleached SYBR Green Light Fluorescent SYBR Green
Bleaching Function -.00078t Flu = e
Bleaching Function -.00068t Flu = e
The Solution • Change R1 in LED Driver from a 4.3 Ohm resistor to a 20 Ohm resistor
The Theory 20 Ohms Optimal Signal Signal 4.3 Ohms R Resistance (Ohms) optimal
Conclusion • Photobleaching significantly affected our system with the 4.3 Ohm resistor • This effect was reduced via increasing the resistance in the LED driver • Further optimization of the system is possible
Acknowledgements Code • Danielle Carpenter, Co-author • Steve Wasserman, Debugging Images • http://distancelearning.ksi.edu/demo/bio378/DNA_files/image023.jpg • http://en.wikivisual.com/images/0/04/NA-comparedto-DNA_thymineAndUracilCorrected.png • http://www.elixa.com/light/arrays2.jpg • http://openwetware.org/wiki/20.309:Measuring_DNA_Melting_Curves