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LECTURES IN MICROBIOLOGY. The Prokaryotes. Sofronio Agustin Professor. LESSON 4. Lesson 4 Topics. External Structures Cell Envelope Internal Structures Cell Shapes, Arrangement, and Sizes Classification. External Structures. Flagella Pili and fimbriae Glycocalyx. Flagella.

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The prokaryotes

LECTURES IN

MICROBIOLOGY

The Prokaryotes

Sofronio Agustin

Professor

LESSON 4


Lesson 4 topics
Lesson 4 Topics

  • External Structures

  • Cell Envelope

  • Internal Structures

  • Cell Shapes, Arrangement, and Sizes

  • Classification


External structures
External Structures

  • Flagella

  • Pili and fimbriae

  • Glycocalyx


Flagella
Flagella

  • Composed of protein subunits called flagellin.

  • “H” antigens used in serotyping of bacterial strains.

  • Example: Escherichia coli O157: H7


Flagellar structure
Flagellar Structure

Three components of a flagellum: filament, hook and basal body


Flagellar arrangement
Flagellar Arrangement

(a) Monotrichous (b) Lophotrichous (c) Amphitrichous (d) Peritrichous


Bacterial motility
Bacterial Motility

The rotation of the flagella enables bacteria to be motile.


Chemotaxis
Chemotaxis

Chemotaxis is the movement of bacteria in response to chemical signals. It consists of a series of tumbles and runs toward or away from source of stimuli.


Endoflagella
Endoflagella

  • Spirochetes have their flagella embedded in the membrane = endoflagella

  • Also called axial filament

  • Example: T. pallidum (corkscrew motility)


Pili and fimbriae
Pili and Fimbriae

  • Attachment

  • Mating (Conjugation)


Fimbriae
Fimbriae

Fimbriae are smaller than flagella and are important for attachment.


Pili

Pili enable conjugation to occur, which is the transfer of DNA from one bacterial cell to another (“mating”).


Glycocalyx
Glycocalyx

  • Capsule

    Protects bacteria from phagocytic cells

  • Slime layer

    Enable attachment and aggregation of bacterial cells


Capsule
Capsule

  • The capsule is covalently bound to the cell wall.

  • Associated with virulence in bacteria.

  • Example:

    Streptococcus pneumoniae


Slime layer
Slime Layer

  • The slime layer is loosely bound to the cell.

  • Carbohydrate rich material enhances adherence of cells on surfaces

  • Example:

    Streptococcus mutans and “plaque formation”


Biofilms
Biofilms

  • The slime layer is associated with cell aggregation and the formation of biofilms

  • Example:

    Staphylococcus epidermidis biofilms on catheter tips


Cell envelope
Cell Envelope

  • Cell wall

    Gram-positive

    Gram-negative

  • Cytoplasmic membrane

  • Cell wall-less bacteria


Cell wall
Cell Wall

  • Gram positive cell wall

    • Thick peptidoglycan (PG) layer

    • Acidic polysaccharides

    • Teichoic acid and lipoteichoic acid

  • Gram-negative cell wall

    • Thin peptidoglycan (PG) layer

    • Lipopolysaccharide layer

    • Porins

    • Periplasmic space


Peptidoglycan layer
Peptidoglycan Layer

PG is a complex sugar and peptide structure important for cell wall stability and shape.


Cell wall structures
Cell Wall Structures

Structures associated with gram-positive and gram-negative cell walls.


Cytoplasmic membrane
Cytoplasmic Membrane

  • Phospholipid bilayer

  • “Fluid mosaic” model

  • Embedded proteins for active transport

  • Enzymes for energy generation

  • Photosynthetic pigments


L forms
L Forms

Mutations can cause some bacteria to lose the ability to synthesize the cell wall and are called L forms.


Cell wall less bacteria
Cell Wall Less Bacteria

  • No peptidoglycan layer

  • Cell membrane contains sterols for stability


The mycoplasma
The Mycoplasma

  • Mycoplasma bacteria have no cell wall, which contributes to their pleomorphic shapes

  • Smallest bacteria

    (0.2 um)

  • Example:

    Mycoplasma pneumoniae

    (SEM on right)


Internal structures
Internal Structures

  • Cytoplasm

  • Genome

  • Inclusion bodies

  • Actin

  • Endospore


Cytoplasm
Cytoplasm

  • Gelatinous solution containing water, nutrients, proteins, and genetic material

  • Site for cell metabolism


Genetic structures
Genetic Structures

  • Deoxyribonucleic acid (DNA)

  • Ribonucleic acid (RNA)

  • Ribosomes


Bacterial genome
Bacterial Genome

Most bacteria contain a single circular double strand of DNA called a nucleoid.


Prokaryotic ribosome
Prokaryotic Ribosome

  • A ribosome is a combination of RNA and protein, and is the site for protein synthesis

  • Composed of large (50S) and small (30S) subunits

  • S = Svedverg unit, measures molecular size


Inclusion bodies
Inclusion Bodies

Inclusion bodies enable a cell to store nutrients and to survive in nutrient depleted environments


Bacterial cytoskeleton
Bacterial Cytoskeleton

Actin is a protein fiber present in some bacteria, which is involved in maintaining cell shape.


Endospores
Endospores

  • Nutrient depletion induces some bacteria (vegetative cell) to form endospores in order to survive

  • Dehydrated gel state due to calcium-protein complex

  • Dipicolinic acid (found only in spores) hardens the spore


Endospore formation
Endospore Formation

Some pathogenic bacteria that produce toxins during the vegetative stage are capable of forming spores. (e.g. Bacillus and Clostridium species)


Bacterial morphology
Bacterial Morphology

  • Coccus

  • Rod or bacillus

  • Curved or spiral

  • Cell arrangements

  • Pleomorphism


Typical shapes and arrangements
Typical Shapes and Arrangements

Scanning electron micrographs of different bacterial shapes and arrangements.

(a) Streptococcus (b) Bacillus (c) Spirochete (d) Spirillum


Pleomorphism
Pleomorphism

Some bacteria show varied shapes and arrangements called pleomorphism. Ex: Corynebacterium diphtheriae’s “Chinese letter” arrangement.


Bacterial shapes and arrangements
Bacterial Shapes and Arrangements

Cellular shapes and arrangements are useful in bacterial identification.


The dimension of bacteria
The Dimension of Bacteria

Relative size of a bacterial cell compared to other cells including viruses.


Classification
Classification

  • Phenotypic methods

  • Molecular methods

  • Taxonomic scheme

  • Unique groups


Phenotypic methods
Phenotypic Methods

  • Cell morphology - staining

  • Biochemical test – enzyme test


Molecular methods
Molecular Methods

  • DNA sequence

  • 16S RNA

  • Protein sequence


Major taxonomic groups of bacteria
Major Taxonomic Groups of Bacteria

  • The methods of classification have allowed bacteria to be classified into different taxonomic groups

  • Re: Bergey’s Manual of Determinative Bacteriology

    (Table on right)


Unique bacterial types
Unique Bacterial Types

  • Intracellular bacteria

  • Photosynthetic bacteria

  • Sulfur bacteria

  • Gliding and fruiting bacteria


Intracellular bacteria
Intracellular Bacteria

  • Intracellular bacteria must live in host cells for them to metabolize and reproduce

  • Examples:

    Rickettsiae

    and Chlamydiae


Cyanobacteria
Cyanobacteria

Cyanobacteria are important photosynthetic bacteria associated with oxygen production.


Sulfur bacteria
Sulfur Bacteria

Green and purple sulfur bacteria are photosynthetic, do not give off oxygen, and are found in sulfur springs, freshwater, and swamps.


Myxobacteria
Myxobacteria

An example of a fruiting body bacteria in which reproductive spores are produced.


Archaea
Archaea

  • Associated with extreme environments

  • Contain unique cell walls

  • Contain unique internal structures


Archaea1
Archaea

  • Archaea are found in:

  • hot springs (thermophiles)

  • high salt content areas (halophiles)

  • Example:

    Halobacterium salinarium


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