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Recipient cell F –

Origin of transfer. F plasmid. Sex pilus. Chromosome. F plasmid transfer. Origin of transfer. The F + donor cell containing an F plasmid is capable of synthesizing a sex pilus. The sex pilus retracts and pulls the donor and recipient cells together. The F plasmid is

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Recipient cell F –

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  1. Origin of transfer F plasmid Sex pilus Chromosome F plasmid transfer Origin of transfer The F+ donor cell containing an F plasmid is capable of synthesizing a sex pilus. The sex pilus retracts and pulls the donor and recipient cells together. The F plasmid is transferred as a single-stranded DNA molecule. + STEP 3 Plasmid transfer Donor cell F+ Recipient cell F– STEP 1Contact The sex pilus contacts the recipient F– cell. The complementary strands to both F plasmid strands are synthesized in the donor and recipient cells. Both cells are F+ and synthesize the sex pilus. STEP 4 Synthesis of a functional plasmid The plasmid is activated for transfer when an endonuclease cleaves one strand of DNA at the origin of transfer. STEP 2 Activation of DNA for transfer F+ cell F+ cell

  2. Origin of transfer F plasmid Sex pilus Chromosome The F+ donor cell containing an F plasmid is capable of synthesizing a sex pilus. + Donor cell F+ Recipient cell F–

  3. Origin of transfer F plasmid Sex pilus Chromosome The F+ donor cell containing an F plasmid is capable of synthesizing a sex pilus. + Donor cell F+ Recipient cell F– STEP 1Contact The sex pilus contacts the recipient F– cell.

  4. Origin of transfer F plasmid Sex pilus Chromosome Origin of transfer The F+ donor cell containing an F plasmid is capable of synthesizing a sex pilus. + Donor cell F+ Recipient cell F– STEP 1Contact The sex pilus contacts the recipient F– cell. The plasmid is activated for transfer when an endonuclease cleaves one strand of DNA at the origin of transfer. STEP 2 Activation of DNA for transfer

  5. Origin of transfer F plasmid Sex pilus Chromosome F plasmid transfer Origin of transfer The F+ donor cell containing an F plasmid is capable of synthesizing a sex pilus. The sex pilus retracts and pulls the donor and recipient cells together. The F plasmid is transferred as a single-stranded DNA molecule. + STEP 3 Plasmid transfer Donor cell F+ Recipient cell F– STEP 1Contact The sex pilus contacts the recipient F– cell. The plasmid is activated for transfer when an endonuclease cleaves one strand of DNA at the origin of transfer. STEP 2 Activation of DNA for transfer

  6. Origin of transfer F plasmid Sex pilus Chromosome F plasmid transfer Origin of transfer The F+ donor cell containing an F plasmid is capable of synthesizing a sex pilus. The sex pilus retracts and pulls the donor and recipient cells together. The F plasmid is transferred as a single-stranded DNA molecule. + STEP 3 Plasmid transfer Donor cell F+ Recipient cell F– STEP 1Contact The sex pilus contacts the recipient F– cell. The complementary strands to both F plasmid strands are synthesized in the donor and recipient cells. Both cells are F+ and synthesize the sex pilus. STEP 4 Synthesis of a functional plasmid The plasmid is activated for transfer when an endonuclease cleaves one strand of DNA at the origin of transfer. STEP 2 Activation of DNA for transfer F+ cell F+ cell

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