What does the word Promoter mean?. It is the place at which RNA Pol II binds. But the word is incorrectly used to describe Enhancers plus Promoter. Initiation by RNA Polymerase II. TFIID recognition site is TATAA. How often is this site found in the genome? 1/4 5.
What does the word Promoter mean?
It is the place at which RNA Pol II binds.
But the word is incorrectly used to describe
Enhancers plus Promoter.
How often is this site found in the genome? 1/45
Once every 1000 nucleotides 109 nucleotides or 106 times
But on a per cell
Basis expression levels of -gal
is about the same
Interrupted Genes in Eukaryotes: Exons and Introns
Most eukaryotic genes contain noncoding sequences called introns that interrupt the coding sequences, or exons. The introns are excised from the RNA transcripts prior to their transport to the cytoplasm.
Removal of Intron Sequences by RNA Splicing
The noncoding introns are excised from gene transcripts by several different mechanisms.
What are Logo plots?
a) Splice acceptor
b) Splice Donor
c) Initiator Met
exon 1 intron 1 exon 2
Proteins are complex macromolecules
composed of 20 (?)
different amino acids.
Protein Synthesis: Translation
The genetic information in mRNA molecules is translated into the amino acid sequences of polypeptides according to the specifications of the genetic code.
Why does one need the ribosome to translate mRNA?
What does “S” mean?
Why do sizes get bigger?
What is Inosine?
No tRNA exists for stop codons!
Dissociation upon finish of protein synthesis
The Genetic Code
The genetic code is a nonoverlapping code, with each amino acid plus polypeptide initiation and termination specified by RNA codons composed of three nucleotides.
You must know single letter codes and some triplets!
Do all cells/animals make the same
Repertoire of tRNAs?
Note it is amber su3…why?????????
If there was a single tRNATyr gene, then could one
have a amber supressor of it?
Was this first????
tRNA environment, protein modifications post-translationally
To Know for Exam
CCATGG (Nco I site and Kozak Rule)
PolyA recog sequence
The Reasons why ATG is a single codon
and TGG is a single codon.