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عنوان آزمايشگاه: اسپكتروفتومتري PowerPoint PPT Presentation


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عنوان آزمايشگاه: اسپكتروفتومتري. هدف آزمايشگاه: آشنايي بااجزاي دستگاه اسپكتروفتومتري وقوانين اسپكتروفتومتري اسپکتروفتومتر ناحیه مریی Spectrophotometer. اسپكتروفتومتر.

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عنوان آزمايشگاه: اسپكتروفتومتري

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Spectrophotometer


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End point . () . - (OD) .


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  • . .

  • .


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  • . . 320 .


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  • . . .

  • . . . .


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  • . . . .


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  • 1- 10 .2- .3- . .4- .5- .6- .


D 20 20

D 20/20

1- . 15 .2- .3- . .4- .5- . . .6- .7- . .


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1- Light Source

2- :

3- (slits)

4- (monochromators)

5- :

6- (

7- ( )


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  • . ( ((Grating system) . .

  • 1- : . 350 800 . (UV) . 200 600 . .


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  • 2- : ( ): . .

  • 3- (slits): : . . . . (band-pass) . Stray light


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  • 4- (monochromators): . . Grating.

  • 5- : (cuvett) . . . 1 cm 1/0 cm 10 . () Single beam Double beam .


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  • 6- ( ): . . . - ( ) nm 495-475 - nm 620-600 . - . .


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  • . .


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  • 7- ( ) . . .


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  • (Flame):

  • . .

  • (Atomic Absorption):

  • (AAS) . .


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-:

  • . I L C ( Ia) ( Ir) ( It) ":

  • It + Ia+Ir= Io


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XC X 100 X=

2-LOG T% = A


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