Crystal Screening and Data Collection Activities at SDC. Jessica Chiu Joint Center for Structural Genomics Stanford Synchrotron Radiation Laboratory JCSG 5 th annual meeting La Jolla, CA, April 6-7,2006. Beamtime provision during SSRL run time. Weekly access to two of the 6 PX beamlines
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Joint Center for Structural Genomics
Stanford Synchrotron Radiation Laboratory
JCSG 5th annual meeting
La Jolla, CA, April 6-7,2006
Weekly access to two of the 6 PX beamlines
All beamlines with automated sample mounting
4 beamlines capable of MAD experiments
Screen ~15,000 crystals per year
Datasets per year
Storage ring upgraded in 2003/4 (SPEAR-3)
Plan to increase from 100mA to 500mA current
2-3 days /
Required during SSRL shutdownperiods (2-3 months per year).
Crystal screening continues at SSRLand pre-screened crystals are taken
to other synchrotron facilities.
Collect ~400datasets per year
Screen ~15000 crystals per year
Fully automated 24/7
crystal screening facility.
Based around X-ray microsource generator.
Temporarily installed in SSRL BL9-2 hutch each shutdown.
Will develop into permanent offline screening facility in 2006
~3 days /
# crystals screened
~21000 crystals screened in total
*The number is lower than expected due to SSRL Spear III
upgrade and safety stand-down at SLAC.
* NMR targets are excluded.
Average screen resolution = 2.46Å
Average collect resolution = 2.31 Å
For 430 crystals/datasets
Average screen resolution = 3.67Å
Average collect resolution = 2.30 Å
For 193 crystals/datasets
*Structures solved are in bold.
Crystal Screening Activity Report
list for every target
Henry van den Bedem
Jinhu Song Craeme Card
Scott McPhillips Clyde Smith
Mathews Irimpan Penjit Moorhead
Ana Gonzalez Thomas Eriksson
NIH Protein Structure Initiative Grant P50 GM62411