IDENTIFYING SNAKE SPECIES INVOLVED IN BITES. New Technology, New Options Disclaimer: I am not involved with any of the companies that make products covered in this presentation. ELISA (Enzyme-linked immunoassay). Inexpensive (relative! c. $275 for 3 reactions) relatively easy to perform
New Technology, New Options
Disclaimer: I am not involved with any of the companies that make products covered in this presentation
LABEL-FREE Kinetic profiling of immunotherapeutics
Vaccine efficacy testing
Eg. BLItzTM system
Drop. Read. Done. Genius!
2. Install a biosensor.
3. Select your assay and click GO. Results appear immediately!
Non-denaturing, minimises non-specific binding
Exceptional specificity even for crude media
Bio-layer interferometry (BLI)
Is this what it takes?
1. Sample collection, storage and extraction
Whatman™ FTA cards:
Collect, archive, transport, and purify nucleic acids at room temperature
minimal handling, streamlined, efficient workflow for sample collection and analysis.
2. Biomarker amplification
Ready-to-go PCR tubes: just add sample (c. $1.50/sample)
Pre-dispensed, room temp stable, reliable and reproducible
Custom formats including primers
Also available in RT-PCR format for single-step amplification from RNA
Still needs a conventional PCR machine (thermal cycler): c. $1700
PCR CYCLER: LavaAmp (prototype)
20-25 reactions max
Powered by 4 x AA batteries or USB cable
$300-$500, aiming for $100
Some development problems delaying it?
3. Vizualisation: FlashGel® System (c. $430, plus c. $10/gel running costs)
5 minute separation and recovery
TOTAL COST PER SAMPLE: $15-17
Equipment: c.£2000 (and can be used for other diagnostics as well