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3 dai 7 dai 11 dai. Control St Control St Control St. 1 2 3 4 5 6. Three biological replicates. Protein extraction. Tryptic digestion and TMT six- plex labeling.
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3 dai 7 dai 11 dai Control St Control St Control St 1 2 3 4 5 6 Three biological replicates Protein extraction Tryptic digestion and TMT six-plexlabeling 1 2 3 4 5 6 126127128129130131 Phosphopeptides enrichment by TiO2 Eluate (Phosphoproteome) Flowthrough (Total proteome) LC-MS/MS Biological replicate 1 Biological replicate 2 Biological replicate 3 One run One run HILIC HILIC One run Two runs Two runs One run Fig. S1 Phosphopeptides and protein identification and validation Bioinformatics analysis
A Stakado Sevin 3 dai 7 dai 11 dai 3 dai 7 dai 11 dai B Stakado Sevin 3 dai 7 dai 11 dai 3 dai 7 dai 11 dai Fig. S2
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