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Célia Regina Monte Barardi celia.barardi@ufsc.br Laboratório de Virologia Aplicada

CORRELATION BETWEEN HUMAN VIRAL CONTAMINATION AND PHYSICOCHEMICAL PROFILES IN A SURFACE WATER LAGOON. Célia Regina Monte Barardi celia.barardi@ufsc.br Laboratório de Virologia Aplicada www.lvapli.ufsc.br Universidade Federal de Santa Catarina, Brasil.

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Célia Regina Monte Barardi celia.barardi@ufsc.br Laboratório de Virologia Aplicada

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  1. CORRELATION BETWEEN HUMAN VIRAL CONTAMINATION AND PHYSICOCHEMICAL PROFILES IN A SURFACE WATER LAGOON

  2. Célia Regina Monte Barardi celia.barardi@ufsc.br Laboratório de Virologia Aplicada www.lvapli.ufsc.br Universidade Federal de Santa Catarina, Brasil

  3. Peri Lagoon: Florianópolis, Santa Catarina, Brazil  Surface area of 5.7 km²  Water is distributed to the inhabitants (110,000 people)  Recreational activities are allowed in the lagoon  More than 2,000 visitors/spring and summer seasons

  4. Introduction • Gastroenteritis, diarrhea, and other diseases can be caused by viruses transmitted by fecal/urino-oral route HAdV – RVA – HAV - JCPyV Introduced into aquatic environments by human – industrial and agricultural activities Studies indicate these viruses as bioindicators of environmental contamination

  5. Objectives • Evaluate the presence of HAdV, JCPyV, HAV and RVA in the largest source of drinking water of the city of Florianópolis, Santa Catarina, Brazil (Peri Lagoon); • Investigate their correlation to the water’s physicochemical parameters and seasonal distribution of these viruses; • Evaluate the integrity and infectivity of detected HAdV. Site 1: Center of the lagoon Site 2: Preserved environment Site 3: Degraded environment Site 4: Where water is collected for treatment and human consumption and also recreational activities

  6. Methods HAdV EnzymaticAssay (Integrity) Plaque AssayandICC-et-RT-qPCR (Infectivity) Font: googleimagens

  7. Results 95.83% - HAdV (46/48) 64.5% - RV-A (31/48) 20.83% - JCPyV (10/48) 12.5% - HAV (6/48) • Viral Distribution • The seasonal distribution Winter: HAdV – HAV - RVA Summer and Spring: JCPyV Site 1: Center of the lagoon Site 2: Preserved environment Site 3: Degraded environment Site 4: Where water is collected for treatment and human consumption and also recreational activities

  8. Results • Correlation: Virus and physicochemical parameters

  9. Results • Integrity and viability of detected HAdV HAdV: Undamaged: 83.3% (10/12) Viability by PA: 66.6% (8/12) Viability by ICC-et-RT-qPCR 83.3% (10/12)

  10. Conclusions • Surface water sources used for human consumption and recreational activities are being contaminated by human enteric viruses; • The divergence among total genomic copy number, genomic copies of intact virus and the number of infectious viruses highlights the importance of including molecular and cell culture techniques during the environmental monitoring of viruses; • ICC-et-RT-qPCR assay was sensible and, fast to detect viral particles infectious (viral mRNA) in water samples; • Emphasizes the urgency of monitoring and implementation efficient methods for removing viral.

  11. Team: Gislaine Fongaro, MSc student Mariana de Almeida do Nascimento, MSc student Aline Viancelli, PhD Denise Tonetta, MSc Maurício Mello Petrucio, PhD Célia Regina Monte Barardi, PhD Financial support: CNPq Project 470808/2009-8 CTAgro/CNPq/- 25/2010 CAPES: Process : AEX 11419/12-1

  12. Lucas ElMahdy Dóris Natália Gislaine Mariana N. Vanessa Mariana R. Camila celia.barardi@ufsc.br Laboratório de Virologia Aplicada www.lvapli.ufsc.br Universidade Federal de Santa Catarina

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