group meeting
Download
Skip this Video
Download Presentation
Group Meeting

Loading in 2 Seconds...

play fullscreen
1 / 16

Group Meeting - PowerPoint PPT Presentation


  • 226 Views
  • Uploaded on

Group Meeting. November 26 th , 2012 Derek Hernandez. Motivation. Method to control topography and chemistry in 3D Derive a better understanding of how these cues can be used to improve migration and alignment in 3D. Chemical Matrix composition Growth factors. Cell behavior Migration

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about ' Group Meeting' - galya


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
group meeting

Group Meeting

November 26th, 2012

Derek Hernandez

motivation
Motivation
  • Method to control topography and chemistry in 3D
  • Derive a better understanding of how these cues can be used to improve migration and alignment in 3D
  • Chemical
  • Matrix composition
  • Growth factors
  • Cell behavior
  • Migration
  • Adhesion
  • Differentiation
  • Proliferation
  • Contact
  • Matrix stiffness
  • Topography
  • Compliance
  • Cellular
  • Junctions
  • Paracrine signals

Lust, JR. University of Rochester, Institute of Optics. Scale bar = 2 µm

protocol to immobilize cues on protein structures
Protocol to immobilize cues on protein structures
  • 1) Fabricate protein structure
  • Concentrated protein solution
  • Photosensitizer
  • High laser intensity
  • 2) Immobilize BP-biotin
  • 2 mg/mL BP-biotin solution
  • Reduced laser intensity

Remove fabrication solution

Protein structure

Benzophenone-biotin

Neutravidin

Biotinylated peptide with PEG linker

3) Bind peptide using neutravidin-biotin chemistry

Remove BP-biotin solution

effect of laser power
Effect of laser power

Functionalization Scans

2 4 6

Scan conditions

2 mg/mL BP-Biotin 10% DMSO

40 mW, 40X objective

0.1 Hz (~30 µm/s)

future work
Future work
  • Focus on limited power range (0-70 mW)
  • Test the effects of:
    • BP-biotin concentration
    • BSA structure density
effect of immobilization on surface topography
Effect of immobilization on surface topography
  • Average roughness of BSA structure is ~100 nm
laser induced shrinking
Laser-induced shrinking

Trying to quantify modulus changes

improving cell interaction with rgd peptide immobilization
Improving cell interaction with RGD peptide immobilization

Cells adhere strongly to and flatten on RGD-functionalized BSA structures

Cells have negative adhesion preferences for unmodified BSA structures

conclusion
Conclusion
  • Cell interaction with structure confined mostly to RGD-functionalized regions

Future Work:

  • Establish a quantifiable metric for cell interaction
  • Use UV excitation to determine target RGD concentration range
  • Use professionally manufactured biotin-RGD-FITC
ad