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Today’s Outline. DNA and DNA Replication Review Leading Strand vs. Lagging Strand DNA Pol I vs. DNA Pol III Telomeres Molecular Sculpting DNA Replication PCR What’s Due? StudyNotes 7 is due on Thursday Mar 21 HW # 5 and is due on Tuesday Mar 26. StudyNotes 8a is due on Tuesday Mar 26.

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Today s outline
Today’s Outline

  • DNA and DNA Replication Review

    • Leading Strand vs. Lagging Strand

    • DNA Pol I vs. DNA Pol III

  • Telomeres

  • Molecular Sculpting

    • DNA Replication

    • PCR

  • What’s Due?

    • StudyNotes 7 is due on Thursday Mar 21

    • HW # 5 and is due on Tuesday Mar 26.

    • StudyNotes 8a is due on Tuesday Mar 26.




5’ carbon

5’ carbon

3’ carbon

3’ carbon



Dna polymerase iii
DNA Polymerase III

Adds dNTPs to the newly synthesized strand on the leading strand. Adds to the 3’ end of a ribose.


Dna polymerase i
DNA Polymerase I

DNA Primase creates primers (red) that are RNA molecules. DNA Pol I replaces the RNA nucleotides with DNA nucleotides.


5

Ends of parental DNA strands

Leading strand

Lagging strand

3

5

3

Last fragment

Previous fragment

Lagging strand

RNA primer

5

3

Parental strand

Removal of primers and replacement with DNA where a 3 end is available

DNA Pol1 Can only add nucleotides to the 3’ end of existing DNA.

5

3

Telomeres protect an organism’s genes from being eroded during successive rounds of DNA replication. In humans the sequence TTAGGG is repeated between 100 and 1000 times.

Second round of replication

5

New leading strand

3

5

New lagging strand

3

Further rounds of replication

Shorter and shorter daughter molecules


Molecular sculpting
Molecular Sculpting

Two Models:

  • DNA Replication – must include:

    • Helicase, topoisomerase, single strand binding proteins, DNA primase, the sliding clamp, DNA pol I, DNA pol III and ligase.

    • Okazaki fragments, RNA primers, leading strand, lagging strand, nucleotides and free dNTPs.

  • Polymerase Chain Reaction – must include:

    • Three panels: denaturing, annealing and extending.

    • DNA, forward and reverse DNA primers, taq polymerase, dNTPs.


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