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Today’s Outline. DNA and DNA Replication Review Leading Strand vs. Lagging Strand DNA Pol I vs. DNA Pol III Telomeres Molecular Sculpting DNA Replication PCR What’s Due? StudyNotes 7 is due on Thursday Mar 21 HW # 5 and is due on Tuesday Mar 26. StudyNotes 8a is due on Tuesday Mar 26.

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Today s outline

Today’s Outline

  • DNA and DNA Replication Review

    • Leading Strand vs. Lagging Strand

    • DNA Pol I vs. DNA Pol III

  • Telomeres

  • Molecular Sculpting

    • DNA Replication

    • PCR

  • What’s Due?

    • StudyNotes 7 is due on Thursday Mar 21

    • HW # 5 and is due on Tuesday Mar 26.

    • StudyNotes 8a is due on Tuesday Mar 26.


Today s outline

hydrogen bonds


Today s outline

Strand


Today s outline

5’ carbon

5’ carbon

3’ carbon

3’ carbon


A dna double helix has a regular repeated structure

A DNA Double Helix has a regular, repeated structure


Dna polymerase iii

DNA Polymerase III

Adds dNTPs to the newly synthesized strand on the leading strand. Adds to the 3’ end of a ribose.


Dna polymerase i

DNA Polymerase I

DNA Primase creates primers (red) that are RNA molecules. DNA Pol I replaces the RNA nucleotides with DNA nucleotides.


Today s outline

5

Ends of parental DNA strands

Leading strand

Lagging strand

3

5

3

Last fragment

Previous fragment

Lagging strand

RNA primer

5

3

Parental strand

Removal of primers and replacement with DNA where a 3 end is available

DNA Pol1 Can only add nucleotides to the 3’ end of existing DNA.

5

3

Telomeres protect an organism’s genes from being eroded during successive rounds of DNA replication. In humans the sequence TTAGGG is repeated between 100 and 1000 times.

Second round of replication

5

New leading strand

3

5

New lagging strand

3

Further rounds of replication

Shorter and shorter daughter molecules


Molecular sculpting

Molecular Sculpting

Two Models:

  • DNA Replication – must include:

    • Helicase, topoisomerase, single strand binding proteins, DNA primase, the sliding clamp, DNA pol I, DNA pol III and ligase.

    • Okazaki fragments, RNA primers, leading strand, lagging strand, nucleotides and free dNTPs.

  • Polymerase Chain Reaction – must include:

    • Three panels: denaturing, annealing and extending.

    • DNA, forward and reverse DNA primers, taq polymerase, dNTPs.


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