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Day 1

(A) Rotarod test. Day 2. Day 3. Day 4. Day 5. Day 1. Test phase Max :32 rpm Acceleration mode (6 repetitions). Training phase 120 s at 5 rpm Constant mode (5 repetitions). (B) MBx and electrophysiology. Week 7. Week 15. Week 23. MBx -16th week. MBx – 24th Week.

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Day 1

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  1. (A) Rotarod test Day 2 Day 3 Day 4 Day 5 Day 1 Test phase Max :32 rpm Acceleration mode (6 repetitions) Training phase 120 s at 5 rpm Constant mode (5 repetitions) (B) MBx and electrophysiology Week 7 Week 15 Week 23 MBx -16th week MBx – 24th Week MBx -8-9th week Supplementary Figure 1 Week 9, 16 & 23 Week 37 Schematic time scale of experiments. A: Rotarod. B: Muscle biopsy (MBx) and electrophysiology. rpm = rotations per minute Electrophysiology

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